Anti-SELS antibody (ab190247)
Key features and details
- Rabbit polyclonal to SELS
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SELS antibody
See all SELS primary antibodies -
Description
Rabbit polyclonal to SELS -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide within Human SELS aa 100 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q9BQE4 -
Positive control
- HeLa, Jurkat and 293T whole cell lysates
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH: 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab190247 was affinity purified using an epitope specific to SELS immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SELS antibody (ab190247)All lanes : Anti-SELS antibody (ab190247) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate in NETN buffer
Lane 2 : HeLa whole cell lysate in RIPA buffer
Lane 3 : 293T whole cell lysate in NETN buffer
Lane 4 : Jurkat whole cell lysate in RIPA buffer
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 21 kDa
Exposure time: 3 minutes
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Immunoprecipitation - Anti-SELS antibody (ab190247)Immunoprecipitation analysis of HeLa cell lysate (in NETN buffer, 0.5 or 1.0 mg per IP reaction; 20% of IP loaded) labeling SELS using ab190247 at 6 μg per reaction (lane 1). a Control IgG was used in lane 2. For blotting immunoprecipitated SELS, ab190247 was used at 1 μg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
