Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PDK1 antibody [EPR13009] - BSA and Azide free (ab249846)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13009] to PDK1 - BSA and Azide free
  • Suitable for: WB, IP
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PDK1 antibody [EPR13009] - BSA and Azide free
    See all PDK1 primary antibodies

  • Description

    Rabbit monoclonal [EPR13009] to PDK1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, HAP1 and LnCaP cell lysates; Human fetal heart lysate.

  • General notes

    ab249846 is the carrier-free version of ab175190 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab249846 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as Mitochondrial Pyruvate dehydrogenase kinase 1

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Images

  • Western blot - Anti-PDK1 antibody [EPR13009] - BSA and Azide free (ab249846)
    Western blot - Anti-PDK1 antibody [EPR13009] - BSA and Azide free (ab249846)
    All lanes : Anti-PDK1 antibody [EPR13009] (ab175190) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : PDK1 knockout HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 49 kDa
    Observed band size: 46 kDa
    why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation (ab175190).

      Lanes 1- 2: Merged signal (red and green). Green - ab175190 observed at 46 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

     ab175190 was shown to react with PDK1 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab264791 (knockout cell lysate ab257164) was used. Wild-type HeLa and PDK1 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab175190 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-PDK1 antibody [EPR13009] - BSA and Azide free (ab249846)
    Western blot - Anti-PDK1 antibody [EPR13009] - BSA and Azide free (ab249846)
    All lanes : Anti-PDK1 antibody [EPR13009] (ab175190) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 cell lysate
    Lane 2 : PDK1 knockout HAP1 cell lysate
    Lane 3 : Human fetal heart lysate
    Lane 4 : LnCaP cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 49 kDa



    This data was developed using the same antibody clone in a different buffer formulation (ab175190).

    Lanes 1 - 4: Merged signal (red and green). Green - ab175190 observed at 46 kDa. Red - loading control, ab8245, observed at 37 kDa.


    ab175190 was shown to specifically recognize PDK1 in wild-type HAP1 cells along with additional cross reactive bands. No band was observed when PDK1 knockout samples were examined. Wild-type and PDK1 knockout samples were subjected to SDS-PAGE. ab175190 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Anti-PDK1 antibody [EPR13009] - BSA and Azide free (ab249846)
    Anti-PDK1 antibody [EPR13009] - BSA and Azide free (ab249846)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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