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Anti-IMPDH2 antibody [EPR8364(B)] (ab131158)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8364(B)] to IMPDH2
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-IMPDH2 antibody [EPR8364(B)]
    See all IMPDH2 primary antibodies

  • Description

    Rabbit monoclonal [EPR8364(B)] to IMPDH2

  • Host species

    Rabbit

  • Specificity

    The immunogen used to raise this antibody shares 79% homology with Human IMPDH1. Cross-reactivity with this protein has not been confirmed experimentally.

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human IMPDH2 aa 100-200. The exact sequence is proprietary.

  • Positive control

    • K562, HepG2, HeLa and NCI-H460 cell lysates; Human colon tissue

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-IMPDH2 antibody [EPR8364(B)] (ab131158)
    Immunocytochemistry/ Immunofluorescence - Anti-IMPDH2 antibody [EPR8364(B)] (ab131158)

    ab131158 staining IMPDH2 in K562 (human chronic myelogenous leukemia) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% PFA and permeabilized with 0.1% triton X-100. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000.

    DAPI was used as a nuclear counterstain and the negative control was PBS only.

  • Western blot - Anti-IMPDH2 antibody [EPR8364(B)] (ab131158)
    Western blot - Anti-IMPDH2 antibody [EPR8364(B)] (ab131158)
    All lanes : Anti-IMPDH2 antibody [EPR8364(B)] (ab131158) at 1/1000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : NCI-H460 cell lysate

    Predicted band size: 56 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IMPDH2 antibody [EPR8364(B)] (ab131158)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IMPDH2 antibody [EPR8364(B)] (ab131158)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling IMPDH2 with ab131158 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-IMPDH2 antibody [EPR8364(B)] (ab131158)
    OI-RD Scanning - Anti-IMPDH2 antibody [EPR8364(B)] (ab131158)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-IMPDH2 antibody [EPR8364(B)] (ab131158)
    Anti-IMPDH2 antibody [EPR8364(B)] (ab131158)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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