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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Other

Anti-MCM5 antibody [EP2682Y] (ab76023)

Anti-MCM5 antibody [EP2682Y] (ab76023)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit monoclonal [EP2682Y] to MCM5
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-MCM5 antibody [EP2682Y]
    See all MCM5 primary antibodies
  • Description

    Rabbit monoclonal [EP2682Y] to MCM5
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human MCM5 aa 100-200 (N terminal). The exact sequence is proprietary.

  • Positive control

    • HeLa, MCF7 and human spleen lysates; human squamous cell cervical carcinoma tissue
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EP2682Y
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Synthesis
    • Other

Images

  • Western blot - Anti-MCM5 antibody [EP2682Y] (ab76023)
    Western blot - Anti-MCM5 antibody [EP2682Y] (ab76023)
    All lanes : Anti-MCM5 antibody [EP2682Y] (ab76023) at 1/2000 dilution

    Lane 1 : Hela cell lysate
    Lane 2 : MCF7 cell lysate
    Lane 3 : Human spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 82 kDa
    Observed band size: 82 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM5 antibody [EP2682Y] (ab76023)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM5 antibody [EP2682Y] (ab76023)

    ab76023, at a 1/100 dilution, staining MCM5 in paraffin embedded human squamous cell cervical carcinoma tissue. A HRP/AP polymerized secondary antibody was used.

    Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM5 antibody [EP2682Y] (ab76023)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM5 antibody [EP2682Y] (ab76023)

    ab76023 showing positive staining in Normal tonsil tissue.

    Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM5 antibody [EP2682Y] (ab76023)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM5 antibody [EP2682Y] (ab76023)

    ab76023 showing negative staining in Normal brain tissue.

    Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM5 antibody [EP2682Y] (ab76023)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM5 antibody [EP2682Y] (ab76023)

    ab76023 showing positive staining in Glioma tissue.

    Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM5 antibody [EP2682Y] (ab76023)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM5 antibody [EP2682Y] (ab76023)

    ab76023 showing positive staining in Colonic adenocarcinoma tissue.

    Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM5 antibody [EP2682Y] (ab76023)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM5 antibody [EP2682Y] (ab76023)

    ab76023 showing positive staining in Endometrial carcinoma tissue.

    Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM5 antibody [EP2682Y] (ab76023)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM5 antibody [EP2682Y] (ab76023)

    ab76023 showing positive staining in Ovarian carcinoma tissue.

    Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-MCM5 antibody [EP2682Y] (ab76023)

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