Anti-MCM5 antibody (ab17967)
Key features and details
- Rabbit polyclonal to MCM5
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MCM5 antibody
See all MCM5 primary antibodies -
Description
Rabbit polyclonal to MCM5 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Orangutan
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Immunogen
Synthetic peptide representing a portion of human minichromosome maintenance deficient 5 encoded within exon 2.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-MCM5 antibody (ab17967)
ab17967, at 0.2
µ g/ml, staining human MCM5 in (3.8, 7.5 or 15µ g for lanes 1, 2 and 3 respectively) HeLa cell lysate by Western blot. ECL detection with 1 minute exposure. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM5 antibody (ab17967)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma (left) and mouse renal cell carcinoma (right) tissues labelling MCM5 with ab17967 at 1/1000 (1µg/ml). Detection: DAB. -
Immunocytochemistry/ Immunofluorescence - Anti-MCM5 antibody (ab17967)ICC/IF image of ab17967 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab17969, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
