Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MCM5 antibody [EP2683Y] (ab75975)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP2683Y] to MCM5
  • Suitable for: ICC, WB, IHC-P, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-MCM5 antibody [EP2683Y]
    See all MCM5 primary antibodies

  • Description

    Rabbit monoclonal [EP2683Y] to MCM5

  • Host species

    Rabbit

  • Specificity

    The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse.

  • Tested applications

    Suitable for: ICC, WB, IHC-P, Flow Cytmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa and MCF7 cell lysates; human squamous cell cervical carcinoma tissue; HeLa cells.

  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EP2683Y

  • Isotype

    IgG

  • Research areas

Images

  • Flow Cytometry - Anti-MCM5 antibody [EP2683Y] (ab75975)
    Flow Cytometry - Anti-MCM5 antibody [EP2683Y] (ab75975)
    Flow Cytometry analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling MCM5 with Purified ab75975 at 1:20 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor ® 488 ,ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
  • Immunocytochemistry - Anti-MCM5 antibody [EP2683Y] (ab75975)
    Immunocytochemistry - Anti-MCM5 antibody [EP2683Y] (ab75975)
    Immunocytochemistry analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling MCM5 with Purified ab75975 at 1:50 dilution (2.1 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488,ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Anti-MCM5 antibody [EP2683Y] (ab75975)
    Anti-MCM5 antibody [EP2683Y] (ab75975)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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