Rat Erythropoietin Antibody Pair - BSA and Azide free (EPO) (ab253420)
Key features and details
- Unconjugated capture and detector antibodies
- Adaptable to any antibody pair-based assay format
- Antibody concentration ~ 1 mg/ml
- BSA and azide free buffer - ready for conjugation
- Reacts with: Rat
Overview
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Product name
Rat Erythropoietin Antibody Pair - BSA and Azide free (EPO)
See all EPO kits -
Assay type
ELISA set -
Range
11.719 pg/ml - 750 pg/ml -
Species reactivity
Reacts with: Rat -
Product overview
The Antibody Pair can be used to quantify Rat Erythropoietin. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.
For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab274398), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.
Download SDS here.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Carrier free
Yes -
Components 10 x 96 tests Rat Erythropoietin (EPO) Capture Antibody (unconjugated) 1 x 100µg Rat Erythropoietin (EPO) Detector Antibody (unconjugated) 1 x 100µg -
Research areas
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Relevance
Human erythropoietin is member of the EPO/TPO family and encodes a secreted, glycosylated cytokine hormone composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types. It is produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals. -
Cellular localization
Secreted -
Alternative names
- EP
- EPO
- EPO alpha
see all -
Database links
- Entrez Gene: 24335 Rat
- SwissProt: P29676 Rat
- Unigene: 11365 Rat
Images
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Sandwich ELISA - Rat Erythropoietin Antibody Pair - BSA and Azide free (EPO) (ab253420)To learn more about the advantages of recombinant antibodies see here.
