Antibodies, Proteins, Kits and Reagents for Life Science

Anti-hnRNP K antibody [EPR10933] - BSA and Azide free (ab249265)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR10933] to hnRNP K - BSA and Azide free
Suitable for: IHC-P, WB
Reacts with: Mouse, Rat, Human

Product name

Anti-hnRNP K antibody [EPR10933] - BSA and Azide free
See all hnRNP K primary antibodies
Description

Rabbit monoclonal [EPR10933] to hnRNP K - BSA and Azide free
Host species

Rabbit
Tested applications

Suitable for: IHC-P, WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IP
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide within Human hnRNP K. The exact sequence is proprietary.
Database link: P61978
General notes
Ab249265 is the carrier-free version of ab156570. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab249265 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Affinity purified
Clonality

Monoclonal
Clone number

EPR10933
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP K antibody [EPR10933] - BSA and Azide free (ab249265)

This data was developed using ab156570, the same antibody clone in a different buffer formulation.
ab156570 showing +ve staining in Human normal tonsil. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP K antibody [EPR10933] - BSA and Azide free (ab249265)

This data was developed using ab156570, the same antibody clone in a different buffer formulation.
ab156570 showing +ve staining in Human thyroid gland carcinoma. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP K antibody [EPR10933] - BSA and Azide free (ab249265)

This data was developed using ab156570, the same antibody clone in a different buffer formulation.ab156570 showing +ve staining in Human normal pancreas. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP K antibody [EPR10933] - BSA and Azide free (ab249265)

This data was developed using ab156570, the same antibody clone in a different buffer formulation.ab156570 showing +ve staining in Human lung adenocarcinoma. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP K antibody [EPR10933] - BSA and Azide free (ab249265)

This data was developed using ab156570, the same antibody clone in a different buffer formulation.ab156570 showing +ve staining in Human urinary bladder carcinoma. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blot - Anti-hnRNP K antibody [EPR10933] - BSA and Azide free (ab249265)

All lanes : Anti-hnRNP K antibody [EPR10933] (ab156570) at 1/1000 dilution

Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 50 kDa
Observed band size: 65 kDa
why is the actual band size different from the predicted?

This data was developed using ab156570, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP K antibody [EPR10933] - BSA and Azide free (ab249265)

This data was developed using ab156570, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human glioma tissue labeling hnRNP K with ab156570 antibody at 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-hnRNP K antibody [EPR10933] - BSA and Azide free (ab249265)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com