Anti-Paxillin (phospho Y31) antibody (ab4832)
Key features and details
- Rabbit polyclonal to Paxillin (phospho Y31)
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Paxillin (phospho Y31) antibody
See all Paxillin primary antibodies -
Description
Rabbit polyclonal to Paxillin (phospho Y31) -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Chicken
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Immunogen
Synthetic peptide (Human) derived from the region of human paxillin that contains tyrosine 31.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using i) a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated paxillin, and ii) a generic tyrosine phosphorylated peptide to remove antibody that is reactive with phosphotyrosine, irrespective of the sequence. The final product is generated by affinity chromatography using a paxillin-derived peptide that is phosphorylated at tyrosine 31. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Paxillin (phospho Y31) antibody (ab4832)
Immunocytochemistry/ Immunofluorescence of NIH/3T3 (mouse embryo fibroblast cell line) treated with 50 ng of PDGF for 10 minutes with ab4832 (1/250). A Goat anti-Rabbit IgG (H+L) Alexa Fluor® 488 conjugate (1/2000) was used as a secondary (Green). DAPI (Blue) was used as a counter stain. F-actin was stained with Alexa Fluor® 555 Rhodamine Phalloidin (1/300). Panel e is untreaded cells and panel f had no primary antibody.
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Western blot - Anti-Paxillin (phospho Y31) antibody (ab4832)All lanes : Anti-Paxillin (phospho Y31) antibody (ab4832)
Lane 1 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate treated for 1 Hr with 100 µM of Pervanadate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate treated for 20 minutes with 50 ng/mL of TNF alpha
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lane 6 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate treated for 10 minutes with 50 ng/mL of PDGF
Lane 7 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 8 : A549 (human lung carcinoma cell line) whole cell lysate treated for 20 minutes with 200 nM of PMA
Lysates/proteins at 20 µl per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) HRP conjugate at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 68 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?ab4832 was diluted between 1/500 and 1/2000.
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Immunocytochemistry/ Immunofluorescence - Anti-Paxillin (phospho Y31) antibody (ab4832)Colocalization of phospho Paxillin and actin in TGF-beta treated NMµMG (mouse mammary) cells. Green = actin stress fibers, Orange = Paxillin [pY31], Yellow = colocalization.
