Anti-Paxillin (phospho Y118) antibody [EPR1903] (ab109547)
![Anti-Paxillin (phospho Y118) antibody [EPR1903] (ab109547)](https://www.abcam.com/ps/products/109/ab109547/Images/ab109547-296748-anti-paxillin-phospho-y118-antibody-epr1903-western-blot.jpg "Anti-Paxillin (phospho Y118) antibody [EPR1903] (ab109547)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1903] to Paxillin (phospho Y118)
- Suitable for: Dot blot, WB
- Reacts with: Human
Overview
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Product name
Anti-Paxillin (phospho Y118) antibody [EPR1903]
See all Paxillin primary antibodies -
Description
Rabbit monoclonal [EPR1903] to Paxillin (phospho Y118) -
Host species
Rabbit -
Specificity
The antibody only detects Paxillin phosphorylated on Tyrosine 118. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
A synthetic phospho-peptide corresponding to residues surrounding Tyrosine 118 of Human Paxillin
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Positive control
- WB: HeLa and Jurkat cell lysates treated with pervanadate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR1903 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Paxillin (phospho Y118) antibody [EPR1903] (ab109547)All lanes : Anti-Paxillin (phospho Y118) antibody [EPR1903] (ab109547) at 1/1000 dilution
Lane 1 : Jurkat (human T cell leukemia T lymphocyte) whole cell lysate
Lane 2 : Jurkat treated with 50mM pervanadate for 5 min whole cell lysate
Lane 3 : Jurkat treated with 50mM pervanadate for 5 min whole cell lysate. Then the membrane was incubated with alkaline phosphatase.
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 65 kDa
Observed band size: 65 kDaBlocking and dilution buffer: 5% NFDM/TBST.
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![Dot Blot - Anti-Paxillin (phospho Y118) antibody [EPR1903] (ab109547)](https://www.abcam.com/ps/products/109/ab109547/Images/ab109547-296736-anti-paxillin-phospho-y118-antibody-epr1903-dot-blot.jpg)
Dot Blot - Anti-Paxillin (phospho Y118) antibody [EPR1903] (ab109547)Dot blot analysis of Paxillin (phospho Y118) phospho peptide (Lane 1) and Paxillin phospho peptide (Lane 2) labelling Paxillin (phospho Y118) with ab109547 at a dilution of 1/1000. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as the secondary antibody at a dilution of 1/20,000. Blocking buffer: 5% NFDM/BST. Dilution buffer: 5% NFDM /TBST.
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![Western blot - Anti-Paxillin (phospho Y118) antibody [EPR1903] (ab109547)](https://www.abcam.com/ps/products/109/ab109547/Images/ab109547-122503-anti-paxillin-phospho-y118-antibody-epr1903-western-blot.jpg)
Western blot - Anti-Paxillin (phospho Y118) antibody [EPR1903] (ab109547)All lanes : Anti-Paxillin (phospho Y118) antibody [EPR1903] (ab109547) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HeLa cell lysate treated with pervanadate
Lysates/proteins at 10 µg per lane.
Predicted band size: 65 kDa
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![Anti-Paxillin (phospho Y118) antibody [EPR1903] (ab109547)](https://www.abcam.com/ps/products/109/ab109547/Images/ab109547-4-benefits-of-recombinant-antibodies.png)
Anti-Paxillin (phospho Y118) antibody [EPR1903] (ab109547)
