Antibodies, Proteins, Kits and Reagents for Life Science

304 886 ₸ Product size

100 µl 304 886 ₸ 10 µl 127 315 ₸

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Anti-Paxillin (phospho Y118) antibody [EPR1903] (ab109547)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR1903] to Paxillin (phospho Y118)
Suitable for: Dot blot, WB
Reacts with: Human

Product name

Anti-Paxillin (phospho Y118) antibody [EPR1903]
See all Paxillin primary antibodies
Description

Rabbit monoclonal [EPR1903] to Paxillin (phospho Y118)
Host species

Rabbit
Specificity

The antibody only detects Paxillin phosphorylated on Tyrosine 118.
Tested Applications & Species

Application Species
WB
Human

See all applications and species data
Immunogen

A synthetic phospho-peptide corresponding to residues surrounding Tyrosine 118 of Human Paxillin
Positive control
- WB: HeLa and Jurkat cell lysates treated with pervanadate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR1903
Isotype

IgG
Research areas

Western blot - Anti-Paxillin (phospho Y118) antibody [EPR1903] (ab109547)

All lanes : Anti-Paxillin (phospho Y118) antibody [EPR1903] (ab109547) at 1/1000 dilution

Lane 1 : Jurkat (human T cell leukemia T lymphocyte) whole cell lysate
Lane 2 : Jurkat treated with 50mM pervanadate for 5 min whole cell lysate
Lane 3 : Jurkat treated with 50mM pervanadate for 5 min whole cell lysate. Then the membrane was incubated with alkaline phosphatase.

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 65 kDa
Observed band size: 65 kDa

Blocking and dilution buffer: 5% NFDM/TBST.
Dot Blot - Anti-Paxillin (phospho Y118) antibody [EPR1903] (ab109547)

Dot blot analysis of Paxillin (phospho Y118) phospho peptide (Lane 1) and Paxillin phospho peptide (Lane 2) labelling Paxillin (phospho Y118) with ab109547 at a dilution of 1/1000. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as the secondary antibody at a dilution of 1/20,000. Blocking buffer: 5% NFDM/BST. Dilution buffer: 5% NFDM /TBST.
Western blot - Anti-Paxillin (phospho Y118) antibody [EPR1903] (ab109547)

All lanes : Anti-Paxillin (phospho Y118) antibody [EPR1903] (ab109547) at 1/1000 dilution

Lane 1 : HeLa cell lysate
Lane 2 : HeLa cell lysate treated with pervanadate

Lysates/proteins at 10 µg per lane.

Predicted band size: 65 kDa
Anti-Paxillin (phospho Y118) antibody [EPR1903] (ab109547)

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