Anti-Paxillin (phospho Y31) antibody - N-terminal (ab229644)
Key features and details
- Rabbit polyclonal to Paxillin (phospho Y31) - N-terminal
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-Paxillin (phospho Y31) antibody - N-terminal
See all Paxillin primary antibodies -
Description
Rabbit polyclonal to Paxillin (phospho Y31) - N-terminal -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide within Human Paxillin (N terminal) (phospho Y31). The exact sequence is proprietary. Conjugated to a protein carrier.
Database link: P49023 -
Positive control
- WB: HeLa whole cell extracts; Pervanadate treated A431 whole cell extracts.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Research areas
Images
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Western blot - Anti-Paxillin (phospho Y31) antibody - N-terminal (ab229644)All lanes : Anti-Paxillin (phospho Y31) antibody - N-terminal (ab229644) at 1/2000 dilution
Lane 1 : Non-transfected HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell extracts
Lane 2 : Paxillin shRNA-transfected HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell extracts
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 65 kDa7.5% SDS-PAGE
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Western blot - Anti-Paxillin (phospho Y31) antibody - N-terminal (ab229644)All lanes : Anti-Paxillin (phospho Y31) antibody - N-terminal (ab229644) at 1/1000 dilution
Lane 1 : Untreated A431 (Human epidermoid carcinoma cell line) whole cell extract
Lane 2 : A431 (Human epidermoid carcinoma cell line) treated with 100 µm pervanadate for 30 minutes, whole cell extract
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 65 kDa7.5% SDS-PAGE
