Antibodies, Proteins, Kits and Reagents for Life Science

328 339 ₸ Product size

100 µl 328 339 ₸

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Anti-RhoGDI antibody [EPR3773] (ab133248)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR3773] to RhoGDI
Suitable for: WB, IHC-P
Knockout validated
Reacts with: Mouse, Human

Product name

Anti-RhoGDI antibody [EPR3773]
See all RhoGDI primary antibodies
Description

Rabbit monoclonal [EPR3773] to RhoGDI
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

A synthetic peptide corresponding to residues within human RhoGDI (P52565).
Positive control
- WB: HEK293T, Jurkat, HeLa and NIH3T3 cell lysates. IHC-P: Human breast carcinoma tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR3773
Isotype

IgG
Research areas

Western blot - Anti-RhoGDI antibody [EPR3773] (ab133248)

All lanes : Anti-RhoGDI antibody [EPR3773] (ab133248) at 1/1000 dilution

Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : ARHGDIA knockout HEK293T cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : HeLa cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

Predicted band size: 23 kDa
Observed band size: 23 kDa

Lanes 1-4: Merged signal (red and green). Green - ab133248 observed at 23 kDa. Red - loading control ab8245 observed at 36 kDa.

ab133248 Anti-RhoGDI antibody [EPR3773] was shown to specifically react with RhoGDI in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266446 (knockout cell lysate ab257355) was used. Wild-type and RhoGDI knockout samples were subjected to SDS-PAGE. ab133248 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RhoGDI antibody [EPR3773] (ab133248)

Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue labelling RhoGDI with ab133248 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blot - Anti-RhoGDI antibody [EPR3773] (ab133248)

All lanes : Anti-RhoGDI antibody [EPR3773] (ab133248) at 1/1000 dilution

Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : ARHGDIA knockout HEK293T cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : HeLa cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

Predicted band size: 23 kDa
Observed band size: 27 kDa
why is the actual band size different from the predicted?

Lanes 1-4: Merged signal (red and green). Green - ab133248 observed at 27 kDa. Red - loading control ab8245 observed at 36 kDa.

ab133248 Anti-RhoGDI antibody [EPR3773] was shown to specifically react with RhoGDI in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266447 (knockout cell lysate ab257356) was used. Wild-type and RhoGDI knockout samples were subjected to SDS-PAGE. ab133248 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-RhoGDI antibody [EPR3773] (ab133248)

All lanes : Anti-RhoGDI antibody [EPR3773] (ab133248) at 1/1000 dilution

Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : NIH3T3 cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 23 kDa
Anti-RhoGDI antibody [EPR3773] (ab133248)

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