Anti-RhoGDI (phospho S174) antibody (ab74142)
Key features and details
- Rabbit polyclonal to RhoGDI (phospho S174)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RhoGDI (phospho S174) antibody
See all RhoGDI primary antibodies -
Description
Rabbit polyclonal to RhoGDI (phospho S174) -
Host species
Rabbit -
Specificity
The antibody detects endogenous levels of RhoGDI only when phosphorylated at serine 174. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthesized phosphopeptide derived from human RhoGDI around the phosphorylation site of serine 174 (R-G-SP-Y-S).
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Positive control
- IHC-P: Human cervix carcinoma tissue. WB: HUVEC cells treated with EGF.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody was purified by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-RhoGDI (phospho S174) antibody (ab74142)All lanes : Anti-RhoGDI (phospho S174) antibody (ab74142) at 1/500 dilution
Lane 1 : HUVEC cells treated with EGF (200ng/ml, 30mins)
Lane 2 : HUVEC cells treated with EGF (200ng/ml, 30mins) with phosphopeptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 23 kDa
Observed band size: 23 kDa
Additional bands at: 105 kDa, 60 kDa. We are unsure as to the identity of these extra bands.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RhoGDI (phospho S174) antibody (ab74142)Human cervix carcinoma tissue stained with ab74142 (1/50 dilution).
1: No phosphopeptide
2: Phosphopeptide
