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Anti-RhoGDI (phospho S174) antibody (ab74142)

Key features and details

  • Rabbit polyclonal to RhoGDI (phospho S174)
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-RhoGDI (phospho S174) antibody
    See all RhoGDI primary antibodies

  • Description

    Rabbit polyclonal to RhoGDI (phospho S174)

  • Host species

    Rabbit

  • Specificity

    The antibody detects endogenous levels of RhoGDI only when phosphorylated at serine 174.

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthesized phosphopeptide derived from human RhoGDI around the phosphorylation site of serine 174 (R-G-SP-Y-S).

  • Positive control

    • IHC-P: Human cervix carcinoma tissue. WB: HUVEC cells treated with EGF.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS

    Without Mg2+ and Ca2+
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Purification notes

    The antibody was purified by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-RhoGDI (phospho S174) antibody (ab74142)
    Western blot - Anti-RhoGDI (phospho S174) antibody (ab74142)
    All lanes : Anti-RhoGDI (phospho S174) antibody (ab74142) at 1/500 dilution

    Lane 1 : HUVEC cells treated with EGF (200ng/ml, 30mins)
    Lane 2 : HUVEC cells treated with EGF (200ng/ml, 30mins) with phosphopeptide at 10 µg

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 23 kDa
    Observed band size: 23 kDa
    Additional bands at: 105 kDa, 60 kDa. We are unsure as to the identity of these extra bands.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RhoGDI (phospho S174) antibody (ab74142)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RhoGDI (phospho S174) antibody (ab74142)
    Human cervix carcinoma tissue stained with ab74142 (1/50 dilution).
    1: No phosphopeptide
    2: Phosphopeptide

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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