Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Periostin antibody [EPR19936] - BSA and Azide free (ab251563)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19936] to Periostin - BSA and Azide free
  • Suitable for: IP, IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-Periostin antibody [EPR19936] - BSA and Azide free
    See all Periostin primary antibodies

  • Description

    Rabbit monoclonal [EPR19936] to Periostin - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251563 is the carrier-free version of ab219057. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251563 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody [EPR19936] - BSA and Azide free (ab251563)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody [EPR19936] - BSA and Azide free (ab251563)
    This data was developed using ab219057, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human colon tissue labeling Periostin with ab219057 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Extracellular matrix staining on human colon (PMID: 26023718). Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody [EPR19936] - BSA and Azide free (ab251563)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody [EPR19936] - BSA and Azide free (ab251563)
    This data was developed using ab219057, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human lung cancer tissue labeling Periostin with ab219057 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Extracellular matrix staining on human lung cancer (PMID: 24273600). Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Western blot - Anti-Periostin antibody [EPR19936] - BSA and Azide free (ab251563)
    Western blot - Anti-Periostin antibody [EPR19936] - BSA and Azide free (ab251563)
    All lanes : Anti-Periostin antibody [EPR19936] (ab219057) at 1/1000 dilution

    Lane 1 : Human colon cancer lysate
    Lane 2 : Human stomach lysate
    Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 4 : Human breast cancer lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 93 kDa
    Observed band size: 93 kDa



    This data was developed using ab219057, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time:ane 1-3: 30 seconds; Lane 4: 5 seconds.

  • Western blot - Anti-Periostin antibody [EPR19936] - BSA and Azide free (ab251563)
    Western blot - Anti-Periostin antibody [EPR19936] - BSA and Azide free (ab251563)
    All lanes : Anti-Periostin antibody [EPR19936] (ab219057) at 1/1000 dilution

    Lane 1 : Human fetal heart lysate
    Lane 2 : Human fetal kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 93 kDa
    Observed band size: 93 kDa


    Exposure time: 30 seconds


    This data was developed using ab219057, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunoprecipitation - Anti-Periostin antibody [EPR19936] - BSA and Azide free (ab251563)
    Immunoprecipitation - Anti-Periostin antibody [EPR19936] - BSA and Azide free (ab251563)
    This data was developed using ab219057, the same antibody clone in a different buffer formulation.Periostin was immunoprecipitated from 0.35 mg of Human fetal heart lysate with ab219057 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab219057 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution. Lane 1: Human fetal heart lysate 10 μg (Input). Lane 2: ab219057 IP in Human fetal heart lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab219057 in Human fetal heart lysate. Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 3 minutes.
  • Anti-Periostin antibody [EPR19936] - BSA and Azide free (ab251563)
    Anti-Periostin antibody [EPR19936] - BSA and Azide free (ab251563)

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