Antibodies, Proteins, Kits and Reagents for Life Science

Key features and details

Mouse monoclonal [2G3] to RhoGDI
Suitable for: WB, Flow Cyt, ICC/IF
Reacts with: Mouse, Rat, Human, African green monkey, Recombinant fragment
Isotype: IgG1

Product name

Anti-RhoGDI antibody [2G3]
See all RhoGDI primary antibodies
Description

Mouse monoclonal [2G3] to RhoGDI
Host species

Mouse

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
WB	Human Recombinant fragment

See all applications and species data

Immunogen

Purified recombinant full length Human RhoGDI (amino acids 1-204) expressed in E. coli.

Run BLAST with Run BLAST with
Positive control
- Human RhoGDI recombinant protein; Jurkat, HeLa, NIH3T3, C6, K562 and COS7 cell lysates; HepG2 and HeLa cells.
General notes

The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term.
Storage buffer

Preservative: 0.05% Sodium azide
Constituent: 99% PBS

Protein stabilizer at 0.5%.
Concentration information loading...
Purity

Protein G purified
Purification notes

Purified from tissue culture supernatant.
Clonality

Monoclonal
Clone number

2G3
Isotype

IgG1
Research areas

Western blot - Anti-RhoGDI antibody [2G3] (ab135252)

Anti-RhoGDI antibody [2G3] (ab135252) at 1/500 dilution + Human RhoGDI recombinant protein.

Predicted band size: 23 kDa
Observed band size: 48.7 kDa
why is the actual band size different from the predicted?

Note: the predicted band size of the full length protein is 23 kDa. The expected band size of the recombinant protein used here is 42.7 kDa.
Western blot - Anti-RhoGDI antibody [2G3] (ab135252)

All lanes : Anti-RhoGDI antibody [2G3] (ab135252) at 1/500 dilution

Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : NIH3T3 cell lysate
Lane 4 : C6 (Rat glial tumor cell line) cell lysate
Lane 5 : K562 cell lysate
Lane 6 : COS-7 (African green monkey kidney fibroblast-like cell line) cell lysate

Predicted band size: 23 kDa
Immunocytochemistry/ Immunofluorescence - Anti-RhoGDI antibody [2G3] (ab135252)

Immunofluorescence analysis of HepG2 cells using ab135252 at 1/200 dilution (green). Blue: DRAQ5 fluorescent DNA dye.
Flow Cytometry - Anti-RhoGDI antibody [2G3] (ab135252)

Flow cytometric analysis of HeLa cells using ab135252 at 1/200 dilution (green) and negative control (red).
Immunocytochemistry/ Immunofluorescence - Anti-RhoGDI antibody [2G3] (ab135252) This image is courtesy of an anonymous Abreview

Immunofluorescence analysis of rat lung epithelial cells, staining RhoGDI with ab135252.

Cells were fixed with paraformaldehyde, permeabilized with 0.2% saponin and blocked with 10% serum for 10 minutes at 20°C. Samples were incubated with primary antibody (1/200 in 10% serum, 0.2% saponin in PBS) for 1 hour at 23°C. An AlexaFluor®488-conjugated goat anti-mouse polyclonal IgG (1/400) was used as the secondary antibody.
See Abreview

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