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Anti-RhoGDI antibody [2G3] (ab135252)

Key features and details

  • Mouse monoclonal [2G3] to RhoGDI
  • Suitable for: WB, Flow Cyt, ICC/IF
  • Reacts with: Mouse, Rat, Human, African green monkey, Recombinant fragment
  • Isotype: IgG1

Overview

  • Product name

    Anti-RhoGDI antibody [2G3]
    See all RhoGDI primary antibodies

  • Description

    Mouse monoclonal [2G3] to RhoGDI

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Human
    Recombinant fragment
    See all applications and species data

  • Immunogen

    Purified recombinant full length Human RhoGDI (amino acids 1-204) expressed in E. coli.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Human RhoGDI recombinant protein; Jurkat, HeLa, NIH3T3, C6, K562 and COS7 cell lysates; HepG2 and HeLa cells.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-RhoGDI antibody [2G3] (ab135252)
    Western blot - Anti-RhoGDI antibody [2G3] (ab135252)
    Anti-RhoGDI antibody [2G3] (ab135252) at 1/500 dilution + Human RhoGDI recombinant protein.

    Predicted band size: 23 kDa
    Observed band size: 48.7 kDa
    why is the actual band size different from the predicted?



    Note: the predicted band size of the full length protein is 23 kDa. The expected band size of the recombinant protein used here is 42.7 kDa.
  • Western blot - Anti-RhoGDI antibody [2G3] (ab135252)
    Western blot - Anti-RhoGDI antibody [2G3] (ab135252)
    All lanes : Anti-RhoGDI antibody [2G3] (ab135252) at 1/500 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : NIH3T3 cell lysate
    Lane 4 : C6 (Rat glial tumor cell line) cell lysate
    Lane 5 : K562 cell lysate
    Lane 6 : COS-7 (African green monkey kidney fibroblast-like cell line) cell lysate

    Predicted band size: 23 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-RhoGDI antibody [2G3] (ab135252)
    Immunocytochemistry/ Immunofluorescence - Anti-RhoGDI antibody [2G3] (ab135252)
    Immunofluorescence analysis of HepG2 cells using ab135252 at 1/200 dilution (green). Blue: DRAQ5 fluorescent DNA dye.
  • Flow Cytometry - Anti-RhoGDI antibody [2G3] (ab135252)
    Flow Cytometry - Anti-RhoGDI antibody [2G3] (ab135252)
    Flow cytometric analysis of HeLa cells using ab135252 at 1/200 dilution (green) and negative control (red).
  • Immunocytochemistry/ Immunofluorescence - Anti-RhoGDI antibody [2G3] (ab135252)
    Immunocytochemistry/ Immunofluorescence - Anti-RhoGDI antibody [2G3] (ab135252) This image is courtesy of an anonymous Abreview
    Immunofluorescence analysis of rat lung epithelial cells, staining RhoGDI with ab135252.

    Cells were fixed with paraformaldehyde, permeabilized with 0.2% saponin and blocked with 10% serum for 10 minutes at 20°C. Samples were incubated with primary antibody (1/200 in 10% serum, 0.2% saponin in PBS) for 1 hour at 23°C. An AlexaFluor®488-conjugated goat anti-mouse polyclonal IgG (1/400) was used as the secondary antibody.

    See Abreview

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