Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ATHL1 antibody [EPR13421] (ab205302)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13421] to ATHL1
  • Suitable for: WB, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-ATHL1 antibody [EPR13421]
    See all ATHL1 primary antibodies

  • Description

    Rabbit monoclonal [EPR13421] to ATHL1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human fetal liver and fetal kidney lysates; BxPC-3 and MCF7 whole cell lysates. ICC/IF: MCF7 and BxPC-3 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-ATHL1 antibody [EPR13421] (ab205302)
    Western blot - Anti-ATHL1 antibody [EPR13421] (ab205302)
    All lanes : Anti-ATHL1 antibody [EPR13421] (ab205302) at 1/1000 dilution

    Lane 1 : Human fetal liver lysate
    Lane 2 : BxPC-3 (Human pancreas adenocarcinoma cells) whole cell lysate
    Lane 3 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 80 kDa
    Observed band size: 80 kDa


    Exposure time: 1 minute


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-ATHL1 antibody [EPR13421] (ab205302)
    Western blot - Anti-ATHL1 antibody [EPR13421] (ab205302)
    Anti-ATHL1 antibody [EPR13421] (ab205302) at 1/1000 dilution + Human fetal kidney lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 80 kDa
    Observed band size: 80 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunocytochemistry/ Immunofluorescence - Anti-ATHL1 antibody [EPR13421] (ab205302)
    Immunocytochemistry/ Immunofluorescence - Anti-ATHL1 antibody [EPR13421] (ab205302)

    Immunofluorescent analysis of 100% Methanol-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling ATHL1 with ab205302 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Confocal image showing cytoplasmic staining on MCF-7 cell line.

    The nuclear counterstain is DAPI (blue).

    Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).

    The negative controls are as follows:-

    -ve control 1: ab205302 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-ATHL1 antibody [EPR13421] (ab205302)
    Immunocytochemistry/ Immunofluorescence - Anti-ATHL1 antibody [EPR13421] (ab205302)

    Immunofluorescent analysis of 100% Methanol-fixed, 0.1% Triton X-100 permeabilized BxPC-3 (Human pancreas adenocarcinoma cells) cells labeling ATHL1 with ab205302 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Confocal image showing cytoplasmic staining on BxPC-3 cell line.

    The nuclear counterstain is DAPI (blue).

    Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).

    The negative controls are as follows:-

    -ve control 1: ab205302 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

  • Anti-ATHL1 antibody [EPR13421] (ab205302)
    Anti-ATHL1 antibody [EPR13421] (ab205302)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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