Glucose Assay Kit (ab272532)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Sample type: Food samples, Other biological fluids, Plasma, Serum
Overview
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Product name
Glucose Assay Kit
See all Glucose kits -
Detection method
Colorimetric -
Sample type
Serum, Plasma, Other biological fluids, Food samples -
Assay type
Quantitative -
Range
39 µM - 16600 µM -
Product overview
Glucose Assay Kit (ab272532) is a simple, direct and automation-ready procedures for measuring glucose concentrations find wide applications in research and drug discovery. This assay is designed to measure glucose directly in serum or plasma without any pretreatment. The improved otoluidine method utilizes a specific color reaction with glucose. The absorbance at 630nm is directly proportional to glucose concentration in the sample.
Sensitive and accurate: Use as little as 5 μL samples. Linear detection range 0.7 mg/dL (39 μM) to 300 mg/dL (16.6 mM) glucose in 96-well plate.
Simple and convenient: The procedure involves addition of a single working reagent and incubation for 8 min in a boiling water bath. Improved reagent stability. The optimized formulation has greatly enhanced the reagent and signal stability.
Low interference in biological samples: No pretreatments are needed. Assays can be directly performed on serum and plasma samples.
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Tested applications
Suitable for: Functional Studiesmore details
Properties
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Storage instructions
Please refer to protocols. -
Components 100 tests Reagent 1 x 50ml Standard 1 x 1ml -
Research areas
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Relevance
Glucose (C6H12O6; FW: 180.16) is a ubiquitous energy source in most organisms, from bacteria to humans. The breakdown of carbohydrates produces mono- and disaccharides, most of which is glucose. Through glycolysis and TCA (citric acid cycle), glucose is oxidized to eventually form CO2 and water, generating the universal energy molecule ATP. Glucose is a primary source of energy for the brain and a critical component in the production of proteins and in lipid metabolism and therefore measurement of glucose level is a key diagnostic parameter for many metabolic disorders.
Images
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Glucose Assay Kit standard curve