Horse Resistin ELISA kit (ab272031)
Key features and details
- Sensitivity: 46 pg/ml
- Range: 46.88 pg/ml - 3000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Horse, Human
Overview
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Product name
Horse Resistin ELISA kit
See all Resistin kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
46 pg/ml -
Range
46.88 pg/ml - 3000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 90.68 71% - 106% Serum 120.9 113% - 132% Plasma 113.6 101% - 134% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Horse, Human -
Product overview
Horse Resistin ELISA Kit is designed for the quantitative determination of Horse Resistin in cell culture supernatants, plasma and serum samples.
This assay employs an antibody specific for Resistin coated on a 96-well plate. Standards and samples are pipetted into the wells and Resistin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Horse Resistin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Resistin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Detects horse and human Resistin.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 100X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml Anti-Horse Resistin coated Microplate (12 x 8 wells) 1 unit Assay Diluent C 1 x 30ml Biotinylated Anti-Horse Resistin Detection Antibody 2 vials Horse Resistin Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Function
Hormone that seems to suppress insulin ability to stimulate glucose uptake into adipose cells. Potentially links obesity to diabetes. -
Tissue specificity
Expressed only in fatty tissues. -
Sequence similarities
Belongs to the resistin/FIZZ family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Adipose tissue specific secretory factor
- Adipose tissue-specific secretory factor
- ADSF
see all -
Database links
- Entrez Gene: 56729 Human
- Omim: 605565 Human
- SwissProt: Q9HD89 Human
- Unigene: 283091 Human
Images
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Horse Resistin Standard Curve.
This standard curve is for demonstration only. A standard curve must be run with each assay.
Horse Resistin ELISA kit (ab272031) Standard curve.