Phospho-ERK2 (T185/Y187) ELISA Kit (ab279793)
Key features and details
- Sample type: Cell Lysate
- Detection method: Colorimetric
- Assay type: Semi-quantitative
- Reacts with: Mouse
Overview
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Product name
Phospho-ERK2 (T185/Y187) ELISA Kit
See all ERK2 kits -
Detection method
Colorimetric -
Sample type
Cell Lysate -
Assay type
Semi-quantitative -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse -
Product overview
Phospho-ERK2 (T185/Y187) ELISA Kit (ab279793) is a very rapid, convenient, and sensitive assay kit that can monitor the activation or function of important biological pathways in mouse cell lysates. By determining phosphorylated ERK2 protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.
This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of mouse phospho-ERK2. An anti-pan ERK2 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and ERK2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and rabbit anti-phospho-ERK2 (T185/Y187) antibody is used to detect phosphorylated ERK2. After washing away unbound antibody, HRP conjugated anti-rabbit IgG is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of ERK2 (T185/Y187) bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 2X Cell lysate buffer 1 x 10ml 5X Assay Diluent 1 x 15ml HRP-conjugated anti-rabbit IgG concentrate (1000X) 1 vial Pan-ERK2 Coated Microplate 1 unit Positive Control - NIH/3T3 cell lysate 1 vial Rabbit anti-phospho-ERK2 (T185/Y187)-antibody 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4) and ARHGEF2.
Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity. -
Sequence similarities
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain. -
Domain
The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases. -
Post-translational
modificationsDually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-187. -
Cellular localization
Nucleus. - Information by UniProt
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Alternative names
- ERK 2
- ERK-2
- ERT1
see all -
Database links
- Entrez Gene: 26413 Mouse
- SwissProt: P63085 Mouse
- Unigene: 196581 Mouse
Images
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Positive Control
NIH/3T3 cells were treated with PDGFBB at 37°C for 20 mins.
Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer.
Serial dilutions of lysates were analyzed in this ELISA.
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NIH/3T3 cells treated with/without PDGF.NIH/3T3 cells were untreated or treated with 50 ng/ml PDGFBB for 10 mins. -
NIH/3T3 cells treated with/without PDGF.NIH/3T3 cells were untreated or treated with 50 ng/ml PDGFBB for 10 mins.
