Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Ndufs1 antibody [EPR11521(B)] (ab169540)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11521(B)] to Ndufs1
  • Suitable for: ICC/IF, WB, IHC-P, IP, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-Ndufs1 antibody [EPR11521(B)]
    See all Ndufs1 primary antibodies

  • Description

    Rabbit monoclonal [EPR11521(B)] to Ndufs1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    within Human Ndufs1 aa 250-350. The exact sequence is proprietary.

  • Positive control

    • Raji, HepG2, HeLa and Jurkat cell lysates. Human cardiac muscle and Human liver tissue. HepG2 and permeabilized Jurkat cells.

  • General notes

    Ndufs1 is a protein encoded by the NDUFS1 gene. The NDUFS1 protein is the largest subunit of complex 1 which is located on the inner mitochondrial membrane. This complex is critical to the oxidative phosphorylation which occurs within the mitochondria.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Ndufs1 antibody [EPR11521(B)] (ab169540)
    Western blot - Anti-Ndufs1 antibody [EPR11521(B)] (ab169540)
    All lanes : Anti-Ndufs1 antibody [EPR11521(B)] (ab169540) at 1/10000 dilution

    Lane 1 : Raji cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 79 kDa
    Observed band size: 75 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs1 antibody [EPR11521(B)] (ab169540)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs1 antibody [EPR11521(B)] (ab169540)

    Immunohistochemical analysis of paraffin embedded Human cardiac muscle tissue labeling Ndufs1 with ab169540 antibody at 1/50.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs1 antibody [EPR11521(B)] (ab169540)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs1 antibody [EPR11521(B)] (ab169540)

    Immunohistochemical analysis of paraffin embedded Human liver tissue labeling Ndufs1 with ab169540 antibody at 1/50.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Ndufs1 antibody [EPR11521(B)] (ab169540)
    Immunocytochemistry/ Immunofluorescence - Anti-Ndufs1 antibody [EPR11521(B)] (ab169540)
    Immunofluorescent analysis of HepG2 cells labeling Ndufs1 with ab169540 at 1/100.
  • Flow Cytometry - Anti-Ndufs1 antibody [EPR11521(B)] (ab169540)
    Flow Cytometry - Anti-Ndufs1 antibody [EPR11521(B)] (ab169540)
    Flow Cytometrical analysis of permeabilized Jurkat cells with ab169540 antibody at a dilution of 1/10 (red) or a rabbit IgG (negative) (green).
  • Immunoprecipitation - Anti-Ndufs1 antibody [EPR11521(B)] (ab169540)
    Immunoprecipitation - Anti-Ndufs1 antibody [EPR11521(B)] (ab169540)
    Immunoprecipitation. ab169540 at 1/10000 staining Ndufs1 in HeLa cell lysate immunoprecipitated using ab169540 at 1/10. Lane 2: Control IgG. Predicted band size : 79 kDa.
  • Anti-Ndufs1 antibody [EPR11521(B)] (ab169540)
    Anti-Ndufs1 antibody [EPR11521(B)] (ab169540)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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