Anti-Ndufs1 antibody [EPR11521(B)] (ab169540)
![Anti-Ndufs1 antibody [EPR11521(B)] (ab169540)](https://www.abcam.com/ps/products/169/ab169540/Images/ab169540-168175-anti-ndufs1-antibody-epr11521b-western-blot.jpg "Anti-Ndufs1 antibody [EPR11521(B)] (ab169540)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11521(B)] to Ndufs1
- Suitable for: ICC/IF, WB, IHC-P, IP, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-Ndufs1 antibody [EPR11521(B)]
See all Ndufs1 primary antibodies -
Description
Rabbit monoclonal [EPR11521(B)] to Ndufs1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIHC-P HumanIP HumanWB Human
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Immunogen
within Human Ndufs1 aa 250-350. The exact sequence is proprietary.
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Positive control
- Raji, HepG2, HeLa and Jurkat cell lysates. Human cardiac muscle and Human liver tissue. HepG2 and permeabilized Jurkat cells.
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General notes
Ndufs1 is a protein encoded by the NDUFS1 gene. The NDUFS1 protein is the largest subunit of complex 1 which is located on the inner mitochondrial membrane. This complex is critical to the oxidative phosphorylation which occurs within the mitochondria.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11521(B) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Ndufs1 antibody [EPR11521(B)] (ab169540)All lanes : Anti-Ndufs1 antibody [EPR11521(B)] (ab169540) at 1/10000 dilution
Lane 1 : Raji cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 79 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs1 antibody [EPR11521(B)] (ab169540)](https://www.abcam.com/ps/products/169/ab169540/Images/ab169540-168173-anti-ndufs1-antibody-epr11521b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs1 antibody [EPR11521(B)] (ab169540)Immunohistochemical analysis of paraffin embedded Human cardiac muscle tissue labeling Ndufs1 with ab169540 antibody at 1/50.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs1 antibody [EPR11521(B)] (ab169540)](https://www.abcam.com/ps/products/169/ab169540/Images/ab169540-168172-anti-ndufs1-antibody-epr11521b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs1 antibody [EPR11521(B)] (ab169540)Immunohistochemical analysis of paraffin embedded Human liver tissue labeling Ndufs1 with ab169540 antibody at 1/50.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-Ndufs1 antibody [EPR11521(B)] (ab169540)](https://www.abcam.com/ps/products/169/ab169540/Images/ab169540-168171-anti-ndufs1-antibody-epr11521b-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Ndufs1 antibody [EPR11521(B)] (ab169540)Immunofluorescent analysis of HepG2 cells labeling Ndufs1 with ab169540 at 1/100. -
![Flow Cytometry - Anti-Ndufs1 antibody [EPR11521(B)] (ab169540)](https://www.abcam.com/ps/products/169/ab169540/Images/ab169540-168170-anti-ndufs1-antibody-epr11521b-flow-cytometry.jpg)
Flow Cytometry - Anti-Ndufs1 antibody [EPR11521(B)] (ab169540)Flow Cytometrical analysis of permeabilized Jurkat cells with ab169540 antibody at a dilution of 1/10 (red) or a rabbit IgG (negative) (green). -
![Immunoprecipitation - Anti-Ndufs1 antibody [EPR11521(B)] (ab169540)](https://www.abcam.com/ps/products/169/ab169540/Images/ab169540-168174-anti-ndufs1-antibody-epr11521b-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Ndufs1 antibody [EPR11521(B)] (ab169540)Immunoprecipitation. ab169540 at 1/10000 staining Ndufs1 in HeLa cell lysate immunoprecipitated using ab169540 at 1/10. Lane 2: Control IgG. Predicted band size : 79 kDa. -
![Anti-Ndufs1 antibody [EPR11521(B)] (ab169540)](https://www.abcam.com/ps/products/169/ab169540/Images/ab169540-7-benefits-of-recombinant-antibodies.png)
Anti-Ndufs1 antibody [EPR11521(B)] (ab169540)
