Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Integrin alpha 4/CD49D antibody [EPR1355Y] - BSA and Azide free (ab239898)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1355Y] to Integrin alpha 4/CD49D - BSA and Azide free
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-Integrin alpha 4/CD49D antibody [EPR1355Y] - BSA and Azide free
    See all Integrin alpha 4/CD49D primary antibodies

  • Description

    Rabbit monoclonal [EPR1355Y] to Integrin alpha 4/CD49D - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab239898 is the carrier-free version of ab81280 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab239898 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.  This product was previously labelled as Integrin alpha 4

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Integrin alpha 4/CD49D antibody [EPR1355Y] - BSA and Azide free (ab239898)
    Western blot - Anti-Integrin alpha 4/CD49D antibody [EPR1355Y] - BSA and Azide free (ab239898)

    This data was developed using ab81280, the same antibody clone in a different buffer formulation.

    Lane 1: Integrin alpha 4 knockout HAP1 cell lysate (20 µg)

    Lane 2: Wild-type HAP1 cell lysate (20 µg)

    Lanes 1 and 2: Merged signal (red and green). Green - ab81280 observed at 150 kDa. Red - loading control, ab8226, observed at 42 kDa.

     

    ab81280 was shown to recognize Integrin alpha 4 in wild-type HAP1 cells along with additional cross-reactive bands. No bands were observed when Integrin alpha 4 knockout samples were used. Wild-type and Integrin alpha 4 knockout samples were subjected to SDS-PAGE. ab81280 and ab8226 (loading control to beta actin) were diluted 1/2000 and 1/1000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1hr at room temperature before imaging.

  • Western blot - Anti-Integrin alpha 4/CD49D antibody [EPR1355Y] - BSA and Azide free (ab239898)
    Western blot - Anti-Integrin alpha 4/CD49D antibody [EPR1355Y] - BSA and Azide free (ab239898)
    All lanes : Anti-Integrin alpha 4/CD49D antibody [EPR1355Y] (ab81280) at 1/1000 dilution

    All lanes : Jurkat whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Performed under non-reducing conditions.

    Predicted band size: 150 kDa
    Observed band size: 150 kDa



    This data was developed using ab81280, the same antibody clone in a different buffer formulation.

    Lanes 1 - 2: Merged signal (red and green). Green - ab81280 observed at 150 kDa. Red - loading control, ab8245, observed at 37 kDa.

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab81280 and ab8245 (loading control) overnight at 4°C. Antibody binding was detected using Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at a 1:10000 dilution for 1hr at room temperature and then imaged.

  • Western blot - Anti-Integrin alpha 4/CD49D antibody [EPR1355Y] - BSA and Azide free (ab239898)
    Western blot - Anti-Integrin alpha 4/CD49D antibody [EPR1355Y] - BSA and Azide free (ab239898)
    Anti-Integrin alpha 4/CD49D antibody [EPR1355Y] (ab81280) at 1/2000 dilution + Jurkat cell lysate at 10 µg

    Secondary
    HRP labelled goat anti rabbit at 1/2000 dilution

    Predicted band size: 150 kDa
    Observed band size: 150 kDa



    This data was developed using ab81280, the same antibody clone in a different buffer formulation.

  • Anti-Integrin alpha 4/CD49D antibody [EPR1355Y] - BSA and Azide free (ab239898)
    Anti-Integrin alpha 4/CD49D antibody [EPR1355Y] - BSA and Azide free (ab239898)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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