Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Histone H3 (acetyl K18) antibody [EPR16595] - BSA and Azide free (ab239414)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16595] to Histone H3 (acetyl K18) - BSA and Azide free
  • Suitable for: WB, ICC/IF, Dot blot, ChIP, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Histone H3 (acetyl K18) antibody [EPR16595] - BSA and Azide free
    See all Histone H3 primary antibodies

  • Description

    Rabbit monoclonal [EPR16595] to Histone H3 (acetyl K18) - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, Dot blot, ChIP, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human colon, Mouse colon and Rat cerebral cortex tissues. ICC/IF: HeLa cells. ChIP: Chromatin prepared from HeLa cells.

  • General notes

    Ab239414 is the carrier-free version of ab177870. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab239414 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: 59% PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR16595

  • Isotype

    IgG

  • Research areas

Images

  • ChIP - Anti-Histone H3 (acetyl K18) antibody [EPR16595] - BSA and Azide free (ab239414)
    ChIP - Anti-Histone H3 (acetyl K18) antibody [EPR16595] - BSA and Azide free (ab239414)

    Chromatin was prepared from HeLa (Human epithelial cells from cervix adenocarcinoma) cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The ChIP was performed with 25μg of chromatin, 2μg of ab177870 (blue), and 20μl of Anti rabbit IgG sepharose beads. 2μg of Rabbit normal IgG was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (SYBR approach). Primers and probes are located in the first kb of the transcribed region.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab177870).
  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (acetyl K18) antibody [EPR16595] - BSA and Azide free (ab239414)
    Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (acetyl K18) antibody [EPR16595] - BSA and Azide free (ab239414)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling Histone H3 (acetyl K18) with ab177870 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/400 dilution (green). Confocal image showing nuclear staining on HeLa cell line. Acetylation levels increased after treatment with Trichostatin A (500 ng/ml) for 4 hours. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (Anti-alpha Tubulin antibody [DM1A] - Loading Control) at 1/500 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) at 1/500 dilution (red).
    The negative controls are as follows;
    -ve control 1: ab177870 at 1/2000 dilution followed by ab150120 at 1/500 dilution.
    -ve control 2: ab7291 at 1/500 dilution followed by ab150077 at 1/400 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab177870).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (acetyl K18) antibody [EPR16595] - BSA and Azide free (ab239414)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (acetyl K18) antibody [EPR16595] - BSA and Azide free (ab239414)

    Immunohistochemical analysis of paraffin-embedded Rat cerebral cortex tissue labeling Histone H3 (acetyl K18) with ab177870 at 1/500 dilution, followed by prediluted Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining on Rat cerebral cortex tissue is observed. Counter stained with Hematoxylin.

    Negative control: Using PBS instead of primary antibody, secondary antibody is prediluted Goat Anti-Rabbit IgG H&L (HRP).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab177870).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (acetyl K18) antibody [EPR16595] - BSA and Azide free (ab239414)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (acetyl K18) antibody [EPR16595] - BSA and Azide free (ab239414)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Histone H3 (acetyl K18) with ab177870 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nucleus staining on glandular epithelium of colon tissue is observed. Counter stained with Hematoxylin.

    Negative control: Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab177870).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Dot Blot - Anti-Histone H3 (acetyl K18) antibody [EPR16595] - BSA and Azide free (ab239414)
    Dot Blot - Anti-Histone H3 (acetyl K18) antibody [EPR16595] - BSA and Azide free (ab239414)

    Dot blot analysis using ab177870 at 1/1000 dilution and Goat Anti-Rabbit IgG H&L (HRP) (ab97051) as secondary antibody at 1/100,000 dilution.

    Lane 1: Histone H3(acetyl K4 + K9 + K14 + K18) peptide

    Lane 2: Histone H3(acetyl K4) peptide

    Lane 3: Histone H3(acetyl K9) peptide

    Lane 4: Histone H3(acetyl K14) peptide

    Lane 5: Histone H3(acetyl K18) peptide

    Lane 6: Histone H3 unmodified peptide

    Blocking and Diluting buffer and concentration: 5% NFDM/TBST

    Exposure time: 10 seconds

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab177870).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (acetyl K18) antibody [EPR16595] - BSA and Azide free (ab239414)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (acetyl K18) antibody [EPR16595] - BSA and Azide free (ab239414)

    Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling Histone H3 (acetyl K18) with ab177870 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nuclear staining on glandular epithelium of mouse colon tissue is observed. Counter stained with Hematoxylin.

    Negative control: Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab177870).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-Histone H3 (acetyl K18) antibody [EPR16595] - BSA and Azide free (ab239414)
    Anti-Histone H3 (acetyl K18) antibody [EPR16595] - BSA and Azide free (ab239414)

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