Astana Biomed Group, an authorized Abcam distributor in Central Asia

Donkey Anti-Goat IgG H&L (Cy5 ®) preadsorbed (ab6566)

Key features and details

  • Donkey polyclonal Secondary Antibody to Goat IgG - H&L (Cy5 ®), pre-adsorbed
  • Conjugation: Cy5 ®. Ex: 650nm, Em: 667nm
  • Host species: Donkey
  • Isotype: IgG
  • Suitable for: ICC, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, WB, ELISA

Overview

  • Product name

    Donkey Anti-Goat IgG H&L (Cy5 ®) preadsorbed
    See all IgG secondary antibodies

  • Description

    Donkey polyclonal Secondary Antibody to Goat IgG - H&L (Cy5 ®), pre-adsorbed

  • Host species

    Donkey

  • Target species

    Goat

  • Tested applications

    Suitable for: ICC, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, WB, ELISAmore details

  • Minimal
    cross-reactivity


    Chicken, Guinea pig, Hamster, Horse, Mouse, Rabbit, Rat more details

  • Immunogen

    Goat IgG, whole molecule

  • Conjugation

    Cy5 ®. Ex: 650nm, Em: 667nm

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 0.88% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis.

  • Conjugation notes

    Cy5.29 (Cyanine 5.29-OSu) (Molecular Weight 975 daltons) Absorption Wavelength: 650 nm Emission Wavelength: 667 nm Fluorochrome/Protein Ratio: 6.0 moles Cy5 per mole of Goat IgG

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • General notes

    This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited. This material is also subject to proprietary rights of GE Healthcare Bio-Sciences Corp. and Carnegie Mellon University and made and sold under License from GE Healthcare Bio-Sciences Corp. This product is licensed for sale only for research. It is NOT licensed for any other use. There is no implied license hereunder for any commercial use. COMMERCIAL USE shall include: 1 Sale, lease, license or other transfer of the material or any material derived or produced from it. 2 Sale, lease, license or other grant of rights to use this material or any material derived or produced from it. 3 Use of this material to perform services for a fee for third parties. If you require a commercial license to use this material and do not have one, please return this material, unopened to Abcam Plc of 330 Cambridge Science Park, Cambridge, CB4 0FL, and any money paid for the material will be refunded.


    This secondaryantibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labeled without unwanted cross reaction.

  • Research areas

Images

  • Immunohistochemistry (Frozen sections) - Donkey Anti-Goat IgG H&L (Cy5 ®) preadsorbed (ab6566)
    Immunohistochemistry (Frozen sections) - Donkey Anti-Goat IgG H&L (Cy5 ®) preadsorbed (ab6566) Li Q et al. ADAM17 is critical for multipolar exit and radial migration of neuronal intermediate progenitor cells in mice cerebral cortex. PLoS One 8:e65703 (2013).

    Embryonic brains were fixed in 4% paraformaldehyde in PBS overnight and placed in 15% sucrose/PBS for 12 h and then 30% sucrose/PBS for 24 h at 4°C. Brains were then embedded in OCT (optimum cutting temperature) mounting medium and froze before sectioning at 10 or 20 µm. Embryonic cerebral cortex from E14.5, E15.5, E16.5 and E18.5 mice were dissected out and sectioned at 10–15 µm for immunohistochemistry experiment with anti-ADAM17 (ab13535) antibody.

    Cy5-labeled donkey anti-goat (ab6566) was used as the secondary antibody (Red).

  • Western blot - Donkey Anti-Goat IgG H&L (Cy5 ®) preadsorbed (ab6566)
    Western blot - Donkey Anti-Goat IgG H&L (Cy5 ®) preadsorbed (ab6566) This image is courtesy of an anonymous Abreview
    Lanes 2-3 : Goat anti-UCP1 at 1/200 dilution

    Lane 1 : Marker
    Lanes 2-3 : Mouse brown adipose tissue lysate at 70 µg

    Secondary
    Lanes 2-3 : Donkey Anti-Goat IgG H&L (Cy5 ®) preadsorbed (ab6566) at 1/2500 dilution

    Observed band size: 33 kDa
    why is the actual band size different from the predicted?

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

© 2021 Astana Biomed Group LLP. All rights reserved.