Human Omentin Antibody Pair - BSA and Azide free (ab270382)
Key features and details
- Unconjugated capture and detector antibodies
- Adaptable to any antibody pair-based assay format
- Antibody concentration ~ 1 mg/ml
- BSA and azide free buffer - ready for conjugation
- Reacts with: Human
Overview
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Product name
Human Omentin Antibody Pair - BSA and Azide free
See all ITLN1 kits -
Assay type
ELISA set -
Range
0.313 pg/ml - 20 pg/ml -
Species reactivity
Reacts with: Human -
Product overview
The Antibody Pair can be used to quantify Human Omentin. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.
For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab269545), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.
Download SDS here.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Carrier free
Yes -
Components 10 x 96 tests Human Omentin Capture Antibody (unconjugated) 1 x 100µg Human Omentin Detector Antibody (unconjugated) 1 x 100µg -
Research areas
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Function
Has no effect on basal glucose uptake but enhances insulin-stimulated glucose uptake in adipocytes. Increases AKT phosphorylation in the absence and presence of insulin. May play a role in the defense system against microorganisms. May specifically recognize carbohydrate chains of pathogens and bacterial components containing galactofuranosyl residues, in a calcium-dependent manner. May be involved in iron metabolism. -
Tissue specificity
Highly expressed in omental adipose tissue where it is found in stromal vascular cells but not in fat cells but is barely detectable in subcutaneous adipose tissue (at protein level). Highly expressed in the small intestine. Also found in the heart, testis, colon, salivary gland, skeletal muscle, pancreas and thyroid and, to a lesser degree, in the uterus, spleen, prostate, lymph node and thymus. -
Sequence similarities
Contains 1 fibrinogen C-terminal domain. -
Developmental stage
Found in fetal small intestine and thymus. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Cell membrane. Secreted. Enriched in lipid rafts. - Information by UniProt
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Alternative names
- Endothelial lectin HL 1
- Endothelial lectin HL-1
- FLJ20022
see all -
Database links
- Entrez Gene: 55600 Human
- Omim: 609873 Human
- SwissProt: Q8WWA0 Human
- Unigene: 50813 Human
Images
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Sandwich ELISA - Human Omentin Antibody Pair - BSA and Azide free (ab270382)To learn more about the advantages of recombinant antibodies see here.
