Anti-KAT8 / MYST1 / MOF antibody (ab72056)
Key features and details
- Rabbit polyclonal to KAT8 / MYST1 / MOF
- Suitable for: IHC-P, WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-KAT8 / MYST1 / MOF antibody
See all KAT8 / MYST1 / MOF primary antibodies -
Description
Rabbit polyclonal to KAT8 / MYST1 / MOF -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cow, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Bat, Elephant
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Immunogen
Synthetic peptide corresponding to Human KAT8/ MYST1/ MOF aa 1-100 (N terminal).
Database link: Q9H7Z6 -
Positive control
- Whole cell lysate from HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-KAT8 / MYST1 / MOF antibody (ab72056)All lanes : Anti-KAT8 / MYST1 / MOF antibody (ab72056) at 0.4 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from HeLa cells at 5 µg
Predicted band size: 52 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Additional bands at: 100 kDa, 19 kDa, 28 kDa. We are unsure as to the identity of these extra bands.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAT8 / MYST1 / MOF antibody (ab72056)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling KAT8 / MYST1 / MOF with ab72056 at 1/1000 (1µg/ml). Detection: DAB. -
Immunoprecipitation - Anti-KAT8 / MYST1 / MOF antibody (ab72056)Detection of Human KAT8 / MYST1 / MOF by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab72056 at 3 µg/mg lysate. Subsequent WB detection was done using ab72056 at 1 µg/ml. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAT8 / MYST1 / MOF antibody (ab72056)IHC image of ab72056 staining in human normal cervix formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab72056, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
