Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-KAT8 / MYST1 / MOF antibody [EPR15803] - BSA and Azide free (ab240355)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15803] to KAT8 / MYST1 / MOF - BSA and Azide free
  • Suitable for: WB, IP, ICC/IF, IHC-P, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-KAT8 / MYST1 / MOF antibody [EPR15803] - BSA and Azide free
    See all KAT8 / MYST1 / MOF primary antibodies

  • Description

    Rabbit monoclonal [EPR15803] to KAT8 / MYST1 / MOF - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab240355 is the carrier-free version of ab200660. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab240355 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Flow Cytometry - Anti-KAT8 / MYST1 / MOF antibody [EPR15803] - BSA and Azide free (ab240355)
    Flow Cytometry - Anti-KAT8 / MYST1 / MOF antibody [EPR15803] - BSA and Azide free (ab240355)

    ab200660 staining KAT8 in the human cell line HepG2 (human hepatocellular carcinoma) by flow cytometry. Cells were fixed with 4% paraformaldehyde and the sample was incubated with the primary antibody at a dilution of 1/200. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.

    Isoytype control: Rabbit monoclonal IgG (Black)

    Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab200660).

  • Immunocytochemistry/ Immunofluorescence - Anti-KAT8 / MYST1 / MOF antibody [EPR15803] - BSA and Azide free (ab240355)
    Immunocytochemistry/ Immunofluorescence - Anti-KAT8 / MYST1 / MOF antibody [EPR15803] - BSA and Azide free (ab240355)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma) cells labeling KAT8 / MYST1 / MOF with ab200660 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing nuclear staining on HepG2 cell line. The nuclear counter stain is DAPI (blue).

    Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/500 dilution (red).
    The negative controls are as follows:
    -ve control 1: ab200660 at 1/2000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary at 1/500 dilution.
    -ve control 2: Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab200660).

  • Immunocytochemistry/ Immunofluorescence - Anti-KAT8 / MYST1 / MOF antibody [EPR15803] - BSA and Azide free (ab240355)
    Immunocytochemistry/ Immunofluorescence - Anti-KAT8 / MYST1 / MOF antibody [EPR15803] - BSA and Azide free (ab240355)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling KAT8 / MYST1 / MOF with ab200660 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing nuclear staining on HeLa cell line. The nuclear counter stain is DAPI (blue).

    Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/500 dilution (red).
    The negative controls are as follows:
    -ve control 1: ab200660 at 1/2000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary at 1/500 dilution.
    -ve control 2: Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab200660).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAT8 / MYST1 / MOF antibody [EPR15803] - BSA and Azide free (ab240355)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAT8 / MYST1 / MOF antibody [EPR15803] - BSA and Azide free (ab240355)

    Immunohistochemical analysis of paraffin-embedded human cervix carcinoma tissue labeling KAT8 / MYST1 / MOF with ab200660 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.

    Nuclear staining on human cervix carcinoma tissue is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab200660).

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAT8 / MYST1 / MOF antibody [EPR15803] - BSA and Azide free (ab240355)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAT8 / MYST1 / MOF antibody [EPR15803] - BSA and Azide free (ab240355)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling KAT8 / MYST1 / MOF with ab200660 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.

    Nuclear staining on rat kidney tissue is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab200660).

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-KAT8 / MYST1 / MOF antibody [EPR15803] - BSA and Azide free (ab240355)
    Immunoprecipitation - Anti-KAT8 / MYST1 / MOF antibody [EPR15803] - BSA and Azide free (ab240355)

    KAT8 / MYST1 / MOF was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate with ab200660 at 1/170 dilution.

    Western blot was performed from the immunoprecipitate using ab200660 at 1/1000 dilution.

    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

    Lane 1: HeLa whole cell lysate 10 µg (Input).

    Lane 2: ab200660 IP in HeLa whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab200660 in HeLa whole cell extract.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab200660).

  • Anti-KAT8 / MYST1 / MOF antibody [EPR15803] - BSA and Azide free (ab240355)
    Anti-KAT8 / MYST1 / MOF antibody [EPR15803] - BSA and Azide free (ab240355)

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