Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab195415)
Key features and details
- Rabbit polyclonal to Histone H3 (acetyl K27) - ChIP Grade
- Suitable for: ChIP-sequencing, WB, ICC/IF, ChIP, Dot blot
- Reacts with: Human, Recombinant fragment
Overview
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Product name
Anti-Histone H3 (acetyl K27) antibody - ChIP Grade
See all Histone H3 primary antibodies -
Description
Rabbit polyclonal to Histone H3 (acetyl K27) - ChIP Grade -
Host species
Rabbit -
Tested applications
Suitable for: ChIP-sequencing, WB, ICC/IF, ChIP, Dot blotmore details -
Species reactivity
Reacts with: Human, Recombinant fragment
Predicted to work with: Mouse, Rat, Arabidopsis thaliana, a wide range of other species
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Immunogen
Synthetic peptide corresponding to Human Histone H3 (acetyl K27) conjugated to keyhole limpet haemocyanin.
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Positive control
- Chromatin from HeLa and K562 cells; whole and histone extracts from HeLa cells; HeLa cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservatives: 0.05% Sodium azide, 0.05% Proclin 300
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Research areas
Images
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ChIP-sequencing - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab195415)
ChIP-seq results obtained with ab195415 directed against Histone H3 (acetyl K27).
ChIP was performed on sheared chromatin from 100,000 K562 cells using 1 µg of ab195415. The IP’d DNA was subsequently analysed on a Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure 2A shows the peak distribution along the complete human X-chromosome. Figure 2B and C show the peak distribution in two regions surrounding the EIF4A2 and GAPDH positive control genes, respectively. The position of the PCR amplicon, used for validating the ChIP assay is indicated with an arrow.
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Dot Blot - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab195415)Dot Blot analysis was performed to test the cross reactivity of ab195415 against Histone H3 (acetyl K27) with peptides containing other histone modifications and the unmodified H3K27. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1/20,000. Figure shows a high specificity of the antibody for the modification of interest.
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ChIP - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab195415)ChIP results obtained with ab195415 directed against Histone H3 (acetyl K27).
ChIP assays were performed using human K562 cells, ab195415 and optimized PCR primer pairs for qPCR. ChIP was performed using sheared chromatin from 100,000 cells. A titration consisting of 0.2, 0.5, 1 and 2 µg of antibody per ChIP experiment was analyzed. IgG (1 µg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the active GAPDH and EIF4A2 genes, used as positive controls, and for the coding regions of the inactive MB and MYT1 genes, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
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ChIP - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab195415)ChIP results obtained with ab195415 directed against Histone H3 (acetyl K27).
ChIP assays were performed using human HeLa cells, ab195415 and optimized PCR primer pairs for qPCR. ChIP was performed using sheared chromatin from 1,000,000 cells. A titration consisting of 1, 2, 5 and 10 µg of antibody per ChIP experiment was analyzed. IgG (2 µg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the active EIF4A2 and ACTB genes, used as positive controls, and for the inactive TSH2B and MYT1 genes, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
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Western blot - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab195415)All lanes : Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab195415) at 1/1000 dilution
Lane 1 : HeLa whole cell extract at 25 µg
Lane 2 : HeLa histone extract at 15 µg
Lane 3 : recombinant histone H2A at 1 µg
Lane 4 : recombinant histone H2B at 1 µg
Lane 5 : recombinant histone H3 at 1 µg
Lane 6 : recombinant histone H4 at 1 µg
Predicted band size: 15 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab195415)Immunofluorescent analysis of HeLa cells labeling Histone H3 (acetyl K27) with ab195415 at 1/500 dilution. Cells were fixed with 4% formaldehyde for 10 minutes and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with ab195415 (top) diluted 1/500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown at the bottom.
