Anti-IL-1 beta antibody (ab9722)
Key features and details
- Rabbit polyclonal to IL-1 beta
- Suitable for: IHC-P, Sandwich ELISA, WB
- Reacts with: Human, Recombinant fragment
- Isotype: unknown
Overview
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Product name
Anti-IL-1 beta antibody
See all IL-1 beta primary antibodies -
Description
Rabbit polyclonal to IL-1 beta -
Host species
Rabbit -
Specificity
Anti-IL-1 beta antibody is expected to react with both the mature and pro form of IL-1 beta.
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Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Recombinant fragment
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Immunogen
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Positive control
- IHC-P: Human adrenal tissue; Recombinant mouse IL-1 beta protein (ab9723) can be used as a positive control in WB
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General notes
This product is no longer batch tested in IHC, for an IHC validated antibody please see ab156791
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Properties
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Form
Lyophilized:Reconstitute with sterile water to 0.1-1.0mg/ml, aliquot and store at -20°C. -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
No preservative, sterile filtered -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
unknown -
Light chain type
unknown -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1 beta antibody (ab9722)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human normal adrenal tissue labelling IL-1 beta with ab9722 at 2 µg/mL (45 minute incubation at room temperature). Heat mediated antigen retrieval was performed using a buffer at pH 6. An HRP-labeled polymer detection system was used with a DAB chromogen.
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Sandwich ELISA - Anti-IL-1 beta antibody (ab9722)Sandwich ELISA detecting IL-1 beta using ab9722 at a concentration of 2 µg/ml.
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Western blot - Anti-IL-1 beta antibody (ab9722)To detect mouse IL-1 beta by Western Blot analysis, ab9722 can be used at a concentration of 0.1-0.2 μg/ml. When used in conjunction with compatible development reagents, the detection limit for recombinant mouse IL-1 beta is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Lanes 1-11: 250, 125, 62.5, 31.25, 15.625, 7.8, 3.9, 1.95, 0.975, 0.4875 and 0.24 ng recombinant mouse IL-1 beta, respectively.
Non-reducing conditions.
