Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

Order now and get it on Wednesday March 10, 2021

Anti-IL-6 antibody [EPR21710] (ab229381)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR21710] to IL-6
Suitable for: WB, IP
Reacts with: Mouse

Product name

Anti-IL-6 antibody [EPR21710]
See all IL-6 primary antibodies
Description

Rabbit monoclonal [EPR21710] to IL-6
Host species

Rabbit
Tested Applications & Species

Application Species
IP
Mouse

WB
Mouse

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: RAW 264.7 treated with LPS and BFA, whole cell lysate. IP: RAW 264.7 treated with LPS and BFA, whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IP	Mouse
WB	Mouse

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR21710
Isotype

IgG
Research areas

Immunoprecipitation - Anti-IL-6 antibody [EPR21710] (ab229381)

IL6 was immunoprecipitated from 0.35 mg of RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) treated with 100 ng/ml Lipopolysaccharide (LPS) for 6 hours, then added 300 ng/ml BFA the last 3 hours, whole cell lysate with ab229381 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab229381 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

Lane 1: RAW 264.7 treated with 100 ng/ml Lipopolysaccharide (LPS) for 6 hours, then added 300 ng/ml BFA the last 3 hours, whole cell lysate 10 µg (Input).
Lane 2: ab229381 IP in RAW 264.7 treated with 100 ng/ml Lipopolysaccharide (LPS) for 6 hours, then added 300 ng/ml BFA the last 3 hours, whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab229381 in RAW 264.7 treated with 100 ng/ml Lipopolysaccharide (LPS) for 6 hours, then added 300 ng/ml BFA the last 3 hours, whole cell lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 2 minutes.
Western blot - Anti-IL-6 antibody [EPR21710] (ab229381)

All lanes : Anti-IL-6 antibody [EPR21710] (ab229381) at 1/1000 dilution

Lane 1 : Untreated RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus), whole cell lysate
Lane 2 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) treated with 100 ng/ml Lipopolysaccharides (LPS) for 6 hours, then added 300 ng/ml BFA for the last 3 hours, whole cell lysate, whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 24 kDa
Observed band size: 24 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.

We detect a non-specific band at approximately 60 KDa.
Anti-IL-6 antibody [EPR21710] (ab229381)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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