Anti-IL-1 beta antibody (ab205924)
Key features and details
- Rabbit polyclonal to IL-1 beta
- Suitable for: WB, IHC-P
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-IL-1 beta antibody
See all IL-1 beta primary antibodies -
Description
Rabbit polyclonal to IL-1 beta -
Host species
Rabbit -
Specificity
ab205924 is primarily directed against mature, 17,000 MW mouse IL-1 beta and is useful in determining its presence in various assays. The antibody does not recognize human IL-1 beta or mouse IL1 alpha based on a neutralization assay. In ELISA formats and other immunoreactive assays, reactivity occurs with rat IL-1 beta. This antibody will recognize 10% of the non-denatured (native) precursor 31,000 MW mouse IL-1 beta containing samples but will primarily detect all of the 17,000 MW mature molecule. However, in immunoblot analysis, the usual procedure of heating the sample in SDS with or without reducing agents will facilitate denaturing of the 31,000 MW IL-1 beta precursor molecule. Denatured 31,000 precursor IL-1 beta will be recognized by this antibody. -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant full length protein corresponding to Mouse IL-1 beta. Prepared by repeated immunizations with recombinant mouse IL-1 beta produced in E.coli.
Database link: P01584
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituents: 0.424% Potassium phosphate, 0.8766% Sodium chloride -
Concentration information loading... -
Purity
DEAE-Chromatography -
Purification notes
This is an IgG preparation of whole rabbit serum purified by DEAE fractionation. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1 beta antibody (ab205924)
Immunohistochemistry of ab205924 in mouse embryonic kidney tissue. Mouse embryonic kidney fixation: FFPE buffered formalin, 10% conc Ag Retrieval: Heat, Citrate pH 6.2. Pressure Cooker. Primary antibody: 2ug/ml for 1.5 hour @ room temperature. Secondary Ab: HRP polymer 1/50 for 45 minutes at room temperature.
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Western blot - Anti-IL-1 beta antibody (ab205924)ab205924 will recognize 10% of the non-denatured (native) precursor 31,000 MW mouse IL-1 beta containing samples but will primarily detect all of the 17,000 MW mature molecule. However, in western blot analysis, the usual procedure of heating the sample in SDS with or without reducing agents will facilitate denaturing of the 31,000 MW IL-1 beta precursor molecule. Denatured IL-1 beta will have a 18 kDa band.
