Anti-xyloglucan antibody [LM15] (ab190146)
![Anti-xyloglucan antibody [LM15] (ab190146)](https://www.abcam.com/ps/products/190/ab190146/Images/ab190146-233945-anti-xyloglucan-antibody-lm15-immunofluorescence.jpg "Anti-xyloglucan antibody [LM15] (ab190146)")
Key features and details
- Rat monoclonal [LM15] to xyloglucan
- Suitable for: ICC/IF
- Reacts with: Plants, Tobacco
- Isotype: IgG2c
Overview
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Product name
Anti-xyloglucan antibody [LM15] -
Description
Rat monoclonal [LM15] to xyloglucan -
Host species
Rat -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Plants, Tobacco
Predicted to work with: Arabidopsis thaliana, Arabidopsis suecica, Arachis hypogaea
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Immunogen
Synthetic peptide corresponding to xyloglucan. Synthetic neoglycoprotein – xylosylated heptasaccharide (XXXG) coupled to BSA. See orginal paper for more information (PubMed: 18498625).
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Positive control
- Tobacco plant cell wall
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General notes
This antibody clone is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
LM15 -
Isotype
IgG2c
Images
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Immunocytochemistry/ Immunofluorescence - Anti-xyloglucan antibody [LM15] (ab190146)
Ab190146 staining Xyloglucan in 10µm thick tobacco plant stem internode sections, after 6 weeks of growth, fixed in 4% formaldehyde.
Sections were treated with and without pectate lysate (to remove pectic homogalacturonan and further expose the LM15 epitope). Non-specific protein-protein interactions were blocked in 3% BSA/0.3M glycine in 0.1%PBS-Tween for 1h. The sections were then incubated with ab190146 at 3μg/ml, for 2h at room temperature. Ab150165, Goat Anti-Rat IgG H&L (Alexa Fluor® 488) preadsorbed, was used as the secondary antibody at 2μg/ml, for 1h at room temperature (shown in green) As expected, the staining is stronger in pectate lysate treated sections than in untreated.
Also included are negative control images (secondary antibody only) for both treatments, demonstrating antibody specificity and low levels of auto-fluorescence.