Anti-FGFR2 antibody [EPR5180] - BSA and Azide free (ab239970)
![Anti-FGFR2 antibody [EPR5180] - BSA and Azide free (ab239970)](https://www.abcam.com/ps/products/239/ab239970/Images/ab239970-1-anti-fgfr2-antibody-epr5180-immunoprecipitation-t47d-human.jpg "Anti-FGFR2 antibody [EPR5180] - BSA and Azide free (ab239970)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5180] to FGFR2 - BSA and Azide free
- Suitable for: IP, WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-FGFR2 antibody [EPR5180] - BSA and Azide free
See all FGFR2 primary antibodies -
Description
Rabbit monoclonal [EPR5180] to FGFR2 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details
Unsuitable for: Flow Cyt,ICC or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IP: T-47D whole cell lysate.
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General notes
Ab239970 is the carrier-free version of ab109372. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab239970 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5180 -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-FGFR2 antibody [EPR5180] - BSA and Azide free (ab239970)
Purified ab109372 at 1/40 dilution (2µg) immunoprecipitating FGFR2 in T-47D whole cell lysate.
Lane 1 (input): T-47D (Human ductal breast epithelial tumor epithelial cell) whole cell lysate 10µg
Lane 2 (+): ab109372 + T-47D whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab109372 in T-47D whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 145 kDa
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109372). -
![Anti-FGFR2 antibody [EPR5180] - BSA and Azide free (ab239970)](https://www.abcam.com/ps/products/239/ab239970/Images/ab239970-2-benefits-of-recombinant-antibodies.png)
Anti-FGFR2 antibody [EPR5180] - BSA and Azide free (ab239970)
