Anti-FGFR2 antibody [SP273] - N-terminal (ab227683)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP273] to FGFR2 - N-terminal
- Suitable for: ELISA, Flow Cyt, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-FGFR2 antibody [SP273] - N-terminal
See all FGFR2 primary antibodies -
Description
Rabbit monoclonal [SP273] to FGFR2 - N-terminal -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Flow Cytometry: Kato III cells. IHC-P: Human stomach adenocarcinoma, colon adenocarcinoma tissue, cervical squamous cell carcinoma, hepatocellular carcinoma, breast ductal carcinoma and bladder transitional cell carcinoma tissues.
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General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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Purity
Protein A/G purified -
Purification notes
Purified from TCS by protein A/G. -
Clonality
Monoclonal -
Clone number
SP273 -
Isotype
IgG -
Research areas
Images
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Formalin-fixed, paraffin-embedded human stomach adenocarcinoma tissue stained for FGFR2 using ab227683 at 1/100 dilution in immunohistochemical analysis.
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Indirect ELISA using ab227683 at varying antibody concentrations (4000~0 ng /ml) and FGFR2 antigen at 1000 ng/ml. Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution dilution was used as a secondary antibody.
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Flow Cytometry analysis of Kato III (human gastric carcinoma cell line) cells labeling FGFR2 with ab227683 at 1/400 dilution (green) compared to a Rabbit IgG negative control (blue).
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Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue stained for FGFR2 using ab227683 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human bladder transitional cell carcinoma tissue stained for FGFR2 using ab227683 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human breast ductal carcinoma tissue stained for FGFR2 using ab227683 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human cervical squamous cell carcinoma tissue stained for FGFR2 using ab227683 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human hepatocellular carcinoma tissue stained for FGFR2 using ab227683 at 1/100 dilution in immunohistochemical analysis.
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