Antibodies, Proteins, Kits and Reagents for Life Science

268 032 ₸ Product size

100 µg 268 032 ₸

Order now and get it on Wednesday March 10, 2021

Key features and details

Mouse monoclonal [IIB5] to BrdU
Suitable for: ICC/IF, Flow Cyt, IHC-Fr, IHC-P
Reacts with: Species independent
Isotype: IgG1

Product name

Anti-BrdU antibody [IIB5]
See all BrdU primary antibodies
Description

Mouse monoclonal [IIB5] to BrdU
Host species

Mouse
Specificity

This antibody also reacts with bromodeoxyuridine when incorporated into nuclear DNA. It cross reacts with iododeoxyuridine (IdU).
Tested applications

Suitable for: ICC/IF, Flow Cyt, IHC-Fr, IHC-Pmore details
Species reactivity

Reacts with: Species independent
Immunogen

Chemical/ Small Molecule conjugated to BSA.
General notes

The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.09% Sodium azide
Constituent: PBS
Concentration information loading...
Purity

Protein G purified
Clonality

Monoclonal
Clone number

IIB5
Myeloma

Sp2/0-Ag14
Isotype

IgG1
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-BrdU antibody [IIB5] (ab8955)

Indirect immunofluorescence staining of BrdU-labeled MR65 lung cancer cells with ab8955.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BrdU antibody [IIB5] (ab8955)

BrdU staining in sponge Halisarca caerulea using ab8955. Metacrylate embedded tissue sections were incubated with 1:200 diluted primary antibody. An avidin-biotin complex kit using biotinylated rabbit anti-mouse antibody and then incubation in avidin-biotin-peroxidase complex was used for detection.
Immunocytochemistry/ Immunofluorescence - Anti-BrdU antibody [IIB5] (ab8955)

Indirect immunofluorescence staining of BrdU-labeled MR65 lung cancer cells using ab8955 (1:1000).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BrdU antibody [IIB5] (ab8955)

BrdU staining in paraffin embedded rat intestine using ab8955. Primary antibody diluted 1:200. Peroxidase-conjugated goat anti mouse secondary antibody was used for detection.
Flow Cytometry - Anti-BrdU antibody [IIB5] (ab8955)

Flow cytometric BrdU-DNA cell cycle analysis in a lung cancer cell line. Dilution 1:100 of primary antibody ab8955. FITC-conjugated goat anti mouse secondary antibody was used for detection.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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