Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-FGFR2 antibody [EPR5180] (ab109372)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5180] to FGFR2
  • Suitable for: WB, IP
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-FGFR2 antibody [EPR5180]
    See all FGFR2 primary antibodies

  • Description

    Rabbit monoclonal [EPR5180] to FGFR2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: MCF7, Jurkat, HeLa, K562, and T47-D cell lysates; IP: T-47D whole cell lysate.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-FGFR2 antibody [EPR5180] (ab109372)
    Western blot - Anti-FGFR2 antibody [EPR5180] (ab109372)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: FGFR2 knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: Jurkat cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab109372 observed at 160 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab109372 was shown to specifically react with FGFR2 when FGFR2 knockout samples were used. Wild-type and FGFR2 knockout samples were subjected to SDS-PAGE.  Ab109372 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10,000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-FGFR2 antibody [EPR5180] (ab109372)
    Western blot - Anti-FGFR2 antibody [EPR5180] (ab109372)
    All lanes : Anti-FGFR2 antibody [EPR5180] (ab109372) at 1/1000 dilution

    Lane 1 : MCF7 cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : K562 cell lysate
    Lane 5 : T47-D cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 92 kDa
    Observed band size: 145 kDa
    why is the actual band size different from the predicted?

  • Immunoprecipitation - Anti-FGFR2 antibody [EPR5180] (ab109372)
    Immunoprecipitation - Anti-FGFR2 antibody [EPR5180] (ab109372)

    Purified ab109372 at 1/40 dilution (2µg) immunoprecipitating FGFR2 in T-47D whole cell lysate.
    Lane 1 (input): T-47D (Human ductal breast epithelial tumor epithelial cell) whole cell lysate 10µg
    Lane 2 (+): ab109372 + T-47D whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab109372 in T-47D whole cell lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 145 kDa

  • Anti-FGFR2 antibody [EPR5180] (ab109372)
    Anti-FGFR2 antibody [EPR5180] (ab109372)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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