Anti-CXCR3 antibody (ab71864)
Key features and details
- Rabbit polyclonal to CXCR3
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CXCR3 antibody
See all CXCR3 primary antibodies -
Description
Rabbit polyclonal to CXCR3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog, Pig
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human CXCR3.
Read Abcam's proprietary immunogen policy (Peptide available as ab74828.) -
Positive control
- This antibody gave a positive signal in the following human tissue lysates: Colon, Small Intestine, Lymph Node and Heart IF/ICC: HeK293 cell line
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab71864 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 41 kDa). ICC/IF Use a concentration of 1 µg/ml. Target
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Function
Receptor for CXCL9, CXCL10 and CXCL11 and mediates the proliferation of human mesangial cells (HMC).
Isoform 2 is a receptor for CXCL4 and also mediates the inhibitory activities of CXCL9, CXCL10 and CXCL11 on the growth of human microvascular endothelial cells (HMVEC). May play a role in angiogenesis.
Isoform 3 mediates the activity of CXCL11. -
Tissue specificity
Isoform 1 and isoform 2 are mainly expressed in heart, kidney, liver and skeletal muscle. Isoform 1 is also expressed in placenta. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 2833 Human
- Entrez Gene: 12766 Mouse
- Entrez Gene: 84475 Rat
- Omim: 300574 Human
- SwissProt: P49682 Human
- SwissProt: O88410 Mouse
- SwissProt: Q9JII9 Rat
- Unigene: 198252 Human
see all -
Alternative names
- C-X-C chemokine receptor type 3 antibody
- CD 183 antibody
- CD182 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-CXCR3 antibody (ab71864)
ICC/IF image of ab71864 stained HEK293 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab71864, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Western blot - Anti-CXCR3 antibody (ab71864)All lanes : Anti-CXCR3 antibody (ab71864) at 1 µg/ml
Lane 1 : Human colon tissue lysate - total protein (ab30051)
Lane 2 : Human small intestine tissue lysate - total protein (ab29276)
Lane 3 : Human lymph node tissue lysate - total protein (ab29871)
Lane 4 : Human heart tissue lysate - total protein (ab29431)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 41 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutesOur observed 70kDa band is supported by the following publications: http://www.jbc.org/content/279/29/30219.long and http://www.jimmunol.org/cgi/content/full/173/10/6234
Protocols
References (2)
ab71864 has been referenced in 2 publications.
- Guan C et al. Antroquinonol Exerts Immunosuppressive Effect on CD8+ T Cell Proliferation and Activation to Resist Depigmentation Induced by H2O2. Oxid Med Cell Longev 2017:9303054 (2017). PubMed: 29456788
- O'Beirne SL et al. CXCL9 Regulates TGF-ß1-Induced Epithelial to Mesenchymal Transition in Human Alveolar Epithelial Cells. J Immunol 195:2788-96 (2015). PubMed: 26268659
Images
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Immunocytochemistry/ Immunofluorescence - Anti-CXCR3 antibody (ab71864)
ICC/IF image of ab71864 stained HEK293 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab71864, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Western blot - Anti-CXCR3 antibody (ab71864)All lanes : Anti-CXCR3 antibody (ab71864) at 1 µg/ml
Lane 1 : Human colon tissue lysate - total protein (ab30051)
Lane 2 : Human small intestine tissue lysate - total protein (ab29276)
Lane 3 : Human lymph node tissue lysate - total protein (ab29871)
Lane 4 : Human heart tissue lysate - total protein (ab29431)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 41 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutesOur observed 70kDa band is supported by the following publications: http://www.jbc.org/content/279/29/30219.long and http://www.jimmunol.org/cgi/content/full/173/10/6234
