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Immunology Innate Immunity Chemokines Alpha Chemokine Rec. (CXCR)

Alexa Fluor® 488 Anti-CXCR4 antibody [UMB2] (ab208128)

Price and availability

348 441 ₸

Availability

Order now and get it on Wednesday March 03, 2021

Alexa Fluor® 488 Anti-CXCR4 antibody [UMB2] (ab208128)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 488 Rabbit monoclonal [UMB2] to CXCR4
  • Suitable for: ICC/IF
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm

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Overview

  • Product name

    Alexa Fluor® 488 Anti-CXCR4 antibody [UMB2]
    See all CXCR4 primary antibodies
  • Description

    Alexa Fluor® 488 Rabbit monoclonal [UMB2] to CXCR4
  • Host species

    Rabbit
  • Conjugation

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm
  • Tested applications

    Suitable for: ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.
    (Peptide available as ab155072)

  • Positive control

    • ICC/IF: Jurkat cells.
  • General notes

    Alternative versions available:
    Anti-CXCR4 antibody [UMB2] (ab124824) - Knockout validated

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    UMB2
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Chemokines
    • Alpha Chemokine Rec. (CXCR)
    • Microbiology
    • Interspecies Interaction
    • Host Virus Interaction
    • Neuroscience
    • Neurology process
    • Growth and Development
    • Axonal Guidance Proteins
    • Stem Cells
    • Neural Stem Cells
    • Surface Molecules
    • Stem Cells
    • Hematopoietic Progenitors
    • Surface Molecules
    • Immunology
    • Adaptive Immunity
    • Regulatory T Cells
    • Stem Cells
    • Endothelial Progenitors
    • Endothelial Markers
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Immunology
    • Immune System Diseases
    • Antiviral Signaling
    • HIV-related
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia

Images

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-CXCR4 antibody [UMB2] (ab208128)
    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-CXCR4 antibody [UMB2] (ab208128)

    ab208128 staining CXCR4 in Jurkat cells. The cells were fixed with 4% formaldehyde (10 min) and then incubated in 1%BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated overnight at +4°C with ab208128 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

     

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

     

    This product also gave a positive signal under the same testing conditions in Jurkat cells fixed with 80% methanol (5 min).

  • Alexa Fluor® 488 Anti-CXCR4 antibody [UMB2] (ab208128)
    Alexa Fluor® 488 Anti-CXCR4 antibody [UMB2] (ab208128)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Alexa Fluor® 488 Anti-CXCR4 antibody [UMB2] (ab208128)

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    Applications: Flow Cyt, ICC/IF, IHC-Fr, IHC-P, WB

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    Applications: Flow Cyt, ICC/IF, IHC-P, WB

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    Applications: Flow Cyt, ICC/IF, IHC-P, WB

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    Anti-CXCR4 antibody - C-terminal (ab219178)

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    Alexa Fluor® 594 Anti-CXCR4 antibody [UMB2] (ab211984)

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