Anti-CXCR4 (phospho S339) antibody (ab74012)
Key features and details
- Rabbit polyclonal to CXCR4 (phospho S339)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CXCR4 (phospho S339) antibody
See all CXCR4 primary antibodies -
Description
Rabbit polyclonal to CXCR4 (phospho S339) -
Host species
Rabbit -
Specificity
ab74012 detects endogenous levels of CXCR4 only when phosphorylated at serine 339 (Mouse: Ser 346). -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic phosphopeptide derived from human CXCR4 around the phosphorylation site of serine 339 (H-S-SP-V-S).
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab74012 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-CXCR4 (phospho S339) antibody (ab74012)All lanes : Anti-CXCR4 (phospho S339) antibody (ab74012) at 1/500 dilution
Lane 1 : Extracts from HUVEC cells
treated with etoposide (25uM, 24hours)
Lane 2 : Extracts from HUVEC cells
treated with etoposide (25uM, 24hours) with the immunising phosphopeptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 40 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCR4 (phospho S339) antibody (ab74012)Immunohistochemistry analysis of paraffin-embedded human brain carcinoma tissue using ab74012, at 1/50 dilution, in the presence (right panel) or absence (left panel) of the immunising phosphopeptide. -
Immunocytochemistry/ Immunofluorescence - Anti-CXCR4 (phospho S339) antibody (ab74012)Immunofluorescence analysis of HeLa cells using ab74012, at 1/500 dilution, in the presence (right panel) or absence (left panel) of the immunising phosphopeptide.
