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Recombinant mouse IL-27-A + EBI3 protein (Fc Chimera Active) (ab214976)

Key features and details

  • Expression system: CHO cells
  • Purity: >= 98% SDS-PAGE
  • Endotoxin level:
  • Active: Yes
  • Suitable for: Functional Studies, SDS-PAGE

Description

  • Product name

    Recombinant mouse IL-27-A + EBI3 protein (Fc Chimera Active)

  • Biological activity

    Bioactivity was measured in a cell proliferation assay of Con A activated mouse splenocytes.

  • Purity

    >= 98 % SDS-PAGE.

  • Endotoxin level

  • Expression system

    CHO cells

  • Accession

    O35228
    Q8K3I6

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Amino Acid Sequence 1

    • Species

      Mouse

    • Sequence

      MSKLLFLSLALWASRSPGYTETALVALSQPRVQCHASRYPVAVDCSWTPL QAPNSTRSTSFIATYRLGVATQQQSQPCLQRSPQASRCTIPDVHLFSTVP YMLNVTAVHPGGASSSLLAFVAERIIKPDPPEGVRLRTAGQRLQVLWHPP ASWPFPDIFSLKYRLRYRRRGASHFRQVGPIEATTFTLRNSKPHAKYCIQ VSAQDLTDYGKPSDWSLPGQVESAPHKP

    • Predicted molecular weight

      25 kDa

    • Amino acids

      1 to 228

    • Additional sequence information

      (NP_056581.1).

    • Amino Acid Sequence 2

    • Species

      Mouse

    • Sequence

      FPTDPLSLQELRREFTVSLYLARKLLSEVQGYVHSFAESRLPGVNLDLLP LGYHLPNVSLTFQAWHHLSDSERLCFLATTLRPFPAMLGGLGTQGTWTSS EREQLWAMRLDLRDLHRHLRFQVLAAGFKCSKEEEDKEEEEEEEEEEKKL PLGALGGPNQVSSQVSWPQLLYTYQLLHSLELVLSRAVRDLLLLSLPRRP GSAWDS

    • Predicted molecular weight

      24 kDa

    • Amino acids

      29 to 234

    • Additional sequence information

      (AAI19403.1).

    • Specifications

    Our Abpromise guarantee covers the use of ab214976 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      Functional Studies

      SDS-PAGE

    • Form

      Lyophilized

    • Additional notes

      The mouse IL-27 complex composed of the Ebi3 subunit (aa 1-228) and the p28 subunit (aa 29-234) is fused through a polypeptide linker to the Fc region of human IgG1.

    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C.

      Constituent: 99% PBS

      Lyophilized from 0.2 µm filtered solution.

      This product is an active protein and may elicit a biological response in vivo, handle with caution.

    • Reconstitution
      Reconstitute at 100 µg/Ll in sterile PBS. Working aliquots are stable for up to 3 months when stored at -20°C.

    General Info

    • Alternative names

      • IL27-A+EBI3

    • Cellular localization

      IL27-A: Secreted. Does not seem to be secreted without coexpression of EBI3. EBI3: Secreted.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab214976? Please let us know so that we can cite the reference in this datasheet.

ab214976 has not yet been referenced specifically in any publications.

Preparation and Storage

  • Alternative names

    • IL27-A+EBI3

  • Cellular localization

    IL27-A: Secreted. Does not seem to be secreted without coexpression of EBI3. EBI3: Secreted.

Images

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