Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-IL-2 antibody [EPR2780] - Low endotoxin, Azide free (ab246689)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2780] to IL-2 - Low endotoxin, Azide free
  • Suitable for: IHC-P, Flow Cyt, WB, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-IL-2 antibody [EPR2780] - Low endotoxin, Azide free
    See all IL-2 primary antibodies

  • Description

    Rabbit monoclonal [EPR2780] to IL-2 - Low endotoxin, Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, Flow Cyt, WB, ICC/IFmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human thymus and PBMC cell lysates, Human tonsil tissue ICC/IF: PBMC cells.

  • General notes

    ab246689 is a carrier-free antibody designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.20
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Purification notes

    Endotoxin level is less than 1 EU/ml as determined by the TAL test.

  • Clonality

    Monoclonal

  • Clone number

    EPR2780

  • Isotype

    IgG

  • Research areas

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-IL-2 antibody [EPR2780] - Low endotoxin, Azide free (ab246689)
    Immunocytochemistry/ Immunofluorescence - Anti-IL-2 antibody [EPR2780] - Low endotoxin, Azide free (ab246689)

    Immunocytochemistry/ Immunofluorescence analysis of PBMC (Human primary peripheral blood mononuclear cell) cells labeling IL-2 with purified ab92381 at 1/500 dilution (4.2 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL) was used as the secondary antibody only control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92381).

  • Flow Cytometry - Anti-IL-2 antibody [EPR2780] - Low endotoxin, Azide free (ab246689)
    Flow Cytometry - Anti-IL-2 antibody [EPR2780] - Low endotoxin, Azide free (ab246689)

    Flow Cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling IL2 with purified ab92381 at 1/100 dilution (10ug/mL) (red). Cells were fixed with 80% methanol and permeabilised using 0.1% Tween-20. A Goat anti rabbit IgG (Alexa Fluor®488) (ab150077) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black) (ab172730). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92381). 

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 antibody [EPR2780] - Low endotoxin, Azide free (ab246689)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 antibody [EPR2780] - Low endotoxin, Azide free (ab246689)

    ab92381 at 1/250 dilution staining IL2 in Formalin-fixed, Paraffin-embedded Human tonsil tissue. Detection: DAB staining.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92381). 

    Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

  • Anti-IL-2 antibody [EPR2780] - Low endotoxin, Azide free (ab246689)
    Anti-IL-2 antibody [EPR2780] - Low endotoxin, Azide free (ab246689)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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