Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-IL-2 antibody [EPR2780] (ab92381)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2780] to IL-2
  • Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-IL-2 antibody [EPR2780]
    See all IL-2 primary antibodies

  • Description

    Rabbit monoclonal [EPR2780] to IL-2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human IL-2 aa 50-150. The exact sequence is proprietary.

  • Positive control

    • WB: Human thymus tissue lysate and PBMC cell lysates. ICC/IF: PBMC cells. IHC-P: Human tonsil tissue. Flow Cyt: Jurkat cells.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR2780

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 antibody [EPR2780] (ab92381)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 antibody [EPR2780] (ab92381)

    ab92381 at 1/250 dilution staining IL2 in Formalin-fixed, Paraffin-embedded Human tonsil tissue. Detection: DAB staining.

    Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-IL-2 antibody [EPR2780] (ab92381)
    Immunocytochemistry/ Immunofluorescence - Anti-IL-2 antibody [EPR2780] (ab92381)

    Immunocytochemistry/ Immunofluorescence analysis of PBMC (Human primary peripheral blood mononuclear cell) cells labeling IL-2 with purified ab92381 at 1/500 dilution (4.2 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL) was used as the secondary antibody only control.

  • Western blot - Anti-IL-2 antibody [EPR2780] (ab92381)
    Western blot - Anti-IL-2 antibody [EPR2780] (ab92381)
    All lanes : Anti-IL-2 antibody [EPR2780] (ab92381) at 1/1000 dilution

    Lane 1 : Human thymus lysate
    Lane 2 : PBMC lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 18 kDa

  • Flow Cytometry - Anti-IL-2 antibody [EPR2780] (ab92381)
    Flow Cytometry - Anti-IL-2 antibody [EPR2780] (ab92381)

    Flow Cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling IL2 with purified ab92381 at 1/100 dilution (10ug/mL) (red). Cells were fixed with 80% methanol and permeabilised using 0.1% Tween-20. A Goat anti rabbit IgG (Alexa Fluor®488) (ab150077) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black) (ab172730). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Anti-IL-2 antibody [EPR2780] (ab92381)
    Anti-IL-2 antibody [EPR2780] (ab92381)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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