Human IL-28B ELISA Kit (ab277438)
Key features and details
- Sensitivity: 0.053 ng/ml
- Range: 0.051 ng/ml - 12.5 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human IL-28B ELISA Kit
See all IL-28B kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% General Inter-assay Sample n Mean SD CV% General -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.053 ng/ml -
Range
0.051 ng/ml - 12.5 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 132.9 112% - 145% Plasma 77.89 75% - 79% Cell culture media 87.64 82% - 96% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human IL-28B ELISA Kit (ab277438) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-28B in serum (human IL-28B concentration is low in normal serum/plasma, and may not be detectable in this assay), plasma and cell culture supernatants.
This assay employs an antibody specific for Human IL-28B coated on a 96-well plate. Standards and samples are pipetted into the wells and Human IL-28B present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human IL-28B antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human IL-28B bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 300X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent C 1 x 30ml Biotinylated Anti-Human IL-28B Antibody 2 vials Anti-Human IL-28B coated Microplate 1 unit Human IL-28B standard protein (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
Cytokine with immunomodulatory activity. Up-regulates MHC class I antigen expression. Displays potent antiviral activity. Also displays antitumor activity. Ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IL28RA. The ligand/receptor complex seems to signal through the Jak-STAT pathway. -
Sequence similarities
Belongs to the IL-28/IL-29 family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Cytokine ZCYTO22
- IFN-lambda-3
- IFN-lambda-4
see all -
Database links
- Entrez Gene: 282617 Human
- Omim: 607402 Human
- SwissProt: Q8IZI9 Human
- Unigene: 406744 Human
Images
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Human IL-28B ELISA kit (ab277438) Standard curve.
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
