Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-IL-18 antibody [EPR19954-188] - BSA and Azide free (ab243295)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19954-188] to IL-18 - BSA and Azide free
  • Suitable for: WB, IHC-P, Flow Cyt
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-IL-18 antibody [EPR19954-188] - BSA and Azide free
    See all IL-18 primary antibodies

  • Description

    Rabbit monoclonal [EPR19954-188] to IL-18 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details
    Unsuitable for: ICC/IF

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human tonsil, liver and kidney tissues. Flow cyt: PC-3 cells.

  • General notes

    Ab243295 is the carrier-free version of ab243091. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab243295 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-IL-18 antibody [EPR19954-188] - BSA and Azide free (ab243295)
    Western blot - Anti-IL-18 antibody [EPR19954-188] - BSA and Azide free (ab243295)
    All lanes : Anti-IL-18 antibody [EPR19954-188] (ab243091) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : IL18 knockout HeLa cell lysate
    Lane 3 : A431 cell lysate
    Lane 4 : Jurkat cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 22 kDa
    Observed band size: 22 kDa



    This data was developed using the same antibody clone in a different buffer formulation (ab243091).

    Lanes 1-4: Merged signal (red and green). Green - ab243091 observed at 22 kDa. Red - loading control ab8245 observed at 37 kDa.  

     ab243091 Anti-IL-18 antibody [EPR19954-188] was shown to specifically react with IL-18 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265274 (knockout cell lysate ab256952) was used. Wild-type and IL-18 knockout samples were subjected to SDS-PAGE. ab243091 and Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (ab52866) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

     

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-18 antibody [EPR19954-188] - BSA and Azide free (ab243295)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-18 antibody [EPR19954-188] - BSA and Azide free (ab243295)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling IL-18 with ab243091 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic and nuclear staining on distal convoluted tubules of human kidney (PMID: 17687255). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243091).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-18 antibody [EPR19954-188] - BSA and Azide free (ab243295)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-18 antibody [EPR19954-188] - BSA and Azide free (ab243295)

    Immunohistochemical analysis of paraffin-embedded human liver tissue labeling IL-18 with ab243091 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on Kupffer cells in human liver (PMID: 19084941). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243091).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-18 antibody [EPR19954-188] - BSA and Azide free (ab243295)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-18 antibody [EPR19954-188] - BSA and Azide free (ab243295)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling IL-18 with ab243091 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on subsets of macrophages in tonsil (PMID: 28842466). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243091).

  • Flow Cytometry - Anti-IL-18 antibody [EPR19954-188] - BSA and Azide free (ab243295)
    Flow Cytometry - Anti-IL-18 antibody [EPR19954-188] - BSA and Azide free (ab243295)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized Jurkat (human T cell leukemia cell line from peripheral blood) (left panel) and PC-3 (human prostate adenocarcinoma cell line) (right panel) cells labeling IL-18 with ab243091 at 1/50 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

    Negative control: Jurkat (PMID 15086390).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243091).

  • Anti-IL-18 antibody [EPR19954-188] - BSA and Azide free (ab243295)
    Anti-IL-18 antibody [EPR19954-188] - BSA and Azide free (ab243295)

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