Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-IL-27-A antibody [EPR18247-86] (ab252216)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18247-86] to IL-27-A
  • Suitable for: WB, Flow Cyt, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-IL-27-A antibody [EPR18247-86]
    See all IL-27-A primary antibodies

  • Description

    Rabbit monoclonal [EPR18247-86] to IL-27-A

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IP
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: RAW 264.7 treated with 100ng/ml LPS for 6h whole cell lysate; THP-1 whole cell lysate. Flow cyt: Daudi and HeLa cells. IP: HeLa whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR18247-86

  • Isotype

    IgG

  • Research areas

Images

  • Immunoprecipitation - Anti-IL-27-A antibody [EPR18247-86] (ab252216)
    Immunoprecipitation - Anti-IL-27-A antibody [EPR18247-86] (ab252216)

    IL-27-A was immunoprecipitated from 0.35 mg HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab252216 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab252216 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

    Lane 1: HeLa whole cell lysate 10 µg (Input).
    Lane 2: ab252216 IP in HeLa whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab252216 in HeLa whole cell lysate.

    Blocking/Dilution buffer: 5% NFDM/TBST.
    Exposure time: 1 minute.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 19620301, PMID: 12121660).

  • Flow Cytometry - Anti-IL-27-A antibody [EPR18247-86] (ab252216)
    Flow Cytometry - Anti-IL-27-A antibody [EPR18247-86] (ab252216)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling IL-27-A with ab252216 at 1/100 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

  • Flow Cytometry - Anti-IL-27-A antibody [EPR18247-86] (ab252216)
    Flow Cytometry - Anti-IL-27-A antibody [EPR18247-86] (ab252216)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized Daudi (human Burkitt's lymphoma lymphoblast cell line) cells labeling IL-27-A with ab252216 at 1/100 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

  • Western blot - Anti-IL-27-A antibody [EPR18247-86] (ab252216)
    Western blot - Anti-IL-27-A antibody [EPR18247-86] (ab252216)
    All lanes : Anti-IL-27-A antibody [EPR18247-86] (ab252216) at 1/1000 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : Daudi (human burkitt's lymphoma lymphoblast) whole cell lysate
    Lane 3 : THP-1 (human monocytic leukemia cell line) whole cell lysate
    Lane 4 : EL4 (mouse lymphoma T lymphocyte) whole cell lysate
    Lane 5 : Rat spleen lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 27 kDa
    Observed band size: 27,48 kDa why is the actual band size different from the predicted?



    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 12121660, PMID: 21385171).

    Blocking/Dilution buffer: 5% NFDM/TBST.

    This blot was developed using a higher sensitivity ECL substrate.

    Exposure times: Lanes 1-3, 5: 3 minutes; Lane 4: 60 seconds.

  • Western blot - Anti-IL-27-A antibody [EPR18247-86] (ab252216)
    Western blot - Anti-IL-27-A antibody [EPR18247-86] (ab252216)
    All lanes : Anti-IL-27-A antibody [EPR18247-86] (ab252216) at 1/1000 dilution

    Lane 1 : Untreated RAW 264.7(mouse Abelson murine leukemia virus-induced tumor macrophage cell line) whole cell lysate
    Lane 2 : RAW 264.7 treated with 100ng/ml LPS for 6h whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 27 kDa
    Observed band size: 27,48 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 12121660, PMID: 21385171)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    This blot was developed using a higher sensitivity ECL substrate.

  • Anti-IL-27-A antibody [EPR18247-86] (ab252216)
    Anti-IL-27-A antibody [EPR18247-86] (ab252216)

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