Anti-IL-18 antibody [EPR22249-212] (ab223293)
![Anti-IL-18 antibody [EPR22249-212] (ab223293)](https://www.abcam.com/ps/products/223/ab223293/Images/ab223293-326953-anti-il-18-antibody-epr22249-212-immunohistochemistry.jpg "Anti-IL-18 antibody [EPR22249-212] (ab223293)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22249-212] to IL-18
- Suitable for: IHC-P
- Reacts with: Mouse, Rat
Overview
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Product name
Anti-IL-18 antibody [EPR22249-212]
See all IL-18 primary antibodies -
Description
Rabbit monoclonal [EPR22249-212] to IL-18 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P MouseRat
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Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Rat and mouse lung and liver tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22249-212 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-18 antibody [EPR22249-212] (ab223293)
Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling IL-18 with ab223293 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on Kupffer cells and hepatic sinus endothelial cells of mouse liver (PMID: 17989120, PMID: 26203299). Countertsained with Heamtoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-18 antibody [EPR22249-212] (ab223293)](https://www.abcam.com/ps/products/223/ab223293/Images/ab223293-326952-anti-il-18-antibody-epr22249-212-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-18 antibody [EPR22249-212] (ab223293)Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling IL-18 with ab223293 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on macrophages of mouse lung (PMID: 17989120). Countertsained with Heamtoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-18 antibody [EPR22249-212] (ab223293)](https://www.abcam.com/ps/products/223/ab223293/Images/ab223293-326951-anti-il-18-antibody-epr22249-212-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-18 antibody [EPR22249-212] (ab223293)Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling IL-18 with ab223293 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on Kupffer cells and hepatic sinus endothelial cells of rat liver (PMID: 17989120, PMID: 26203299). Countertsained with Heamtoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-18 antibody [EPR22249-212] (ab223293)](https://www.abcam.com/ps/products/223/ab223293/Images/ab223293-326950-anti-il-18-antibody-epr22249-212-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-18 antibody [EPR22249-212] (ab223293)Immunohistochemical analysis of paraffin-embedded rat lung tissue labeling IL-18 with ab223293 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on macrophages of rat lung (PMID: 17989120). Countertsained with Heamtoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Anti-IL-18 antibody [EPR22249-212] (ab223293)](https://www.abcam.com/ps/products/223/ab223293/Images/ab223293-5-benefits-of-recombinant-antibodies.png)
Anti-IL-18 antibody [EPR22249-212] (ab223293)
