Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-KIF1A antibody [EPR11790(B)] - BSA and Azide free (ab240222)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11790(B)] to KIF1A - BSA and Azide free
  • Suitable for: WB, Flow Cyt, IP, ICC/IF
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-KIF1A antibody [EPR11790(B)] - BSA and Azide free
    See all KIF1A primary antibodies

  • Description

    Rabbit monoclonal [EPR11790(B)] to KIF1A - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cyt, IP, ICC/IFmore details
    Unsuitable for: IHC-P

  • Species reactivity

    Reacts with: Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab240222 is the carrier-free version of ab180153. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab240222 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunoprecipitation - Anti-KIF1A antibody [EPR11790(B)] - BSA and Azide free (ab240222)
    Immunoprecipitation - Anti-KIF1A antibody [EPR11790(B)] - BSA and Azide free (ab240222)

    ab180153 (purified) at 1/50 dilution (2ug) immunoprecipitating KIF1A in SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate.

    Lane 1 (input): SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate 10ug

    Lane 2 (+): ab180153 + SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate 10ug

    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab180153 in SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate

    For western blotting, ab131366 VeriBlot for IP Detection Reagent (HRP) was used for detection at 1/10000 dilution. 

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab180153).

  • Flow Cytometry - Anti-KIF1A antibody [EPR11790(B)] - BSA and Azide free (ab240222)
    Flow Cytometry - Anti-KIF1A antibody [EPR11790(B)] - BSA and Azide free (ab240222)

    Flow Cytometry analysis of SH-SY5Y (Human neuroblastoma cell line from bone marrow) cells labeling KIF1A with purified ab180153 at 1/90 dilution (10 ug/ml). Cells were fixed with 4% paraformaldehyde. A goat anti-rabbit IgG (Alexa Fluor® 488) was used as the secondary antibody at 1/2000 dilution. Black - Isotype control, Rabbit monoclonal IgG. Blue - unlabelled control, cells without incubation with primary antibody and secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab180153).

  • Immunocytochemistry/ Immunofluorescence - Anti-KIF1A antibody [EPR11790(B)] - BSA and Azide free (ab240222)
    Immunocytochemistry/ Immunofluorescence - Anti-KIF1A antibody [EPR11790(B)] - BSA and Azide free (ab240222)

    Immunocytochemistry/Immunofluorescence analysis of U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cells labeling KIF1A with purified ab180153 at 1/100 dilution (9.2μg/ml). Cells were fixed in 4% paraformaldehyde and permeabilised with 0.1% tritonX-100. Ab195889, anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used as the secondary antibody at 1/200 dilution (2.5 μg/ml). DAPI was used as the nuclear counter stain.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab180153).

  • Immunoprecipitation - Anti-KIF1A antibody [EPR11790(B)] - BSA and Azide free (ab240222)
    Immunoprecipitation - Anti-KIF1A antibody [EPR11790(B)] - BSA and Azide free (ab240222)

    Western blot analysis of 293 cell lysate immunoprecipitated using unpurified ab180153 at 1/30 dilution, followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab180153).

  • Flow Cytometry - Anti-KIF1A antibody [EPR11790(B)] - BSA and Azide free (ab240222)
    Flow Cytometry - Anti-KIF1A antibody [EPR11790(B)] - BSA and Azide free (ab240222)

    Flow cytometric analysis of 2% paraformaldehyde-fixed SH-SY5Y cells labeling KIF1A with unpurified ab180153 at 1/150 dilution (red), compared to a Rabbit IgG control (green), followed by Goat anti rabbit IgG (FITC) at 1/75 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab180153).

  • Immunocytochemistry/ Immunofluorescence - Anti-KIF1A antibody [EPR11790(B)] - BSA and Azide free (ab240222)
    Immunocytochemistry/ Immunofluorescence - Anti-KIF1A antibody [EPR11790(B)] - BSA and Azide free (ab240222)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed 293 cells labeling KIF1A with unpurified ab180153 at 1/100 dilution, followed by Goat anti rabbit IgG (Dylight 555) at 1/250 dilution. Counter stained with Dapi.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab180153).

  • Anti-KIF1A antibody [EPR11790(B)] - BSA and Azide free (ab240222)
    Anti-KIF1A antibody [EPR11790(B)] - BSA and Azide free (ab240222)

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