Anti-S1P antibody (ab140592)
Key features and details
- Rabbit polyclonal to S1P
- Suitable for: WB
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
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Product name
Anti-S1P antibody
See all S1P primary antibodies -
Description
Rabbit polyclonal to S1P -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat
Predicted to work with: Rabbit, Horse, Chicken, Human, Pig, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster, Orangutan
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Immunogen
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Positive control
- WB: Mouse and rat liver tissue lysates.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab140592 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 118 kDa).Can be blocked with Human S1P peptide (ab223083). Target
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Relevance
S1P (also known as MBTPS1,SKI1), was first discovered as one of two enzymes that cleave the sterol regulatory binding proteins (SREBPs), in the case of S1P at “site-1,” a hydrophillic loop extending into the lumen of the ER. The MBTPS1 domain structure contains a signal sequence followed by a propeptide domain, a catalytic domain, a transmembrane domain and a cytoplasmic domain. -
Cellular localization
Endoplasmic reticulum membrane; Single-pass type I membrane protein. Golgi apparatus membrane; Single-pass type I membrane protein. -
Database links
- Entrez Gene: 100689417 Chinese hamster
- Entrez Gene: 8720 Human
- Entrez Gene: 56453 Mouse
- Entrez Gene: 89842 Rat
- Omim: 603355 Human
- SwissProt: Q9Z2A8 Chinese hamster
- SwissProt: Q14703 Human
- SwissProt: Q9WTZ2 Mouse
see all -
Alternative names
- kexin isozyme 1 antibody
- KIAA0091 antibody
- MBTPS1 antibody
see all
Images
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Western blot - Anti-S1P antibody (ab140592)All lanes : Anti-S1P antibody (ab140592) at 1 µg/ml
Lane 1 : Mouse liver tissue lysate
Lane 2 : Rat liver tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 118 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Additional bands at: 62 kDa (possible non-specific binding)
Exposure time: 90 secondsThe band observed at 100 kDa could potentially be a cleaved form of S1P due to the presence of a 17 amino acid signal peptide and a 169 amino acid pro-peptide.
This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab140592 overnight at 4°C.
Datasheets and documents
References (2)
ab140592 has been referenced in 2 publications.
- Chen R et al. Dabigatran Suppresses PAR-1/SphK/S1P Activation of Astrocytes in Experimental Autoimmune Encephalomyelitis Model. Front Mol Neurosci 13:114 (2020). PubMed: 32694981
- Guo C et al. Cholesterol Homeostatic Regulator SCAP-SREBP2 Integrates NLRP3 Inflammasome Activation and Cholesterol Biosynthetic Signaling in Macrophages. Immunity 49:842-856.e7 (2018). PubMed: 30366764
Images
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Western blot - Anti-S1P antibody (ab140592)All lanes : Anti-S1P antibody (ab140592) at 1 µg/ml
Lane 1 : Mouse liver tissue lysate
Lane 2 : Rat liver tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 118 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Additional bands at: 62 kDa (possible non-specific binding)
Exposure time: 90 secondsThe band observed at 100 kDa could potentially be a cleaved form of S1P due to the presence of a 17 amino acid signal peptide and a 169 amino acid pro-peptide.
This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab140592 overnight at 4°C.
