Anti-OXCT1/SCOT antibody (ab224250)
Key features and details
- Rabbit polyclonal to OXCT1/SCOT
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-OXCT1/SCOT antibody
See all OXCT1/SCOT primary antibodies -
Description
Rabbit polyclonal to OXCT1/SCOT -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Pig -
Immunogen
Recombinant fragment corresponding to Human OXCT1/SCOT aa 50-250.
Database link: P55809 -
Positive control
- WB: HeLa, NIH/3T3 and NBT-II cell lysates; human liver, heart, testis and kidney tissue lysates. IHC-P: Human heart muscle tissue. ICC/IF: A431 cells.
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General notes
Protein previously labeled as OXCT1.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol, PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Paraffin-embedded human kidney stained for OXCT1 using ab224250 at 1/200 dilution in immunohistochemical analysis. Shows strong cytoplasmic positivity in cells in tubules.
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Paraffin-embedded human testis stained for OXCT1 using ab224250 at 1/200 dilution in immunohistochemical analysis. Shows strong cytoplasmic positivity in cells in seminiferous ducts.
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Paraffin-embedded human heart stained for OXCT1 using ab224250 at 1/200 dilution in immunohistochemical analysis. Shows strong cytoplasmic positivity in cardiomyocytes.
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Paraffin-embedded human liver stained for OXCT1 using ab224250 at 1/200 dilution in immunohistochemical analysis. Shows no positivity in hepatocytes as expected.
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Lane 1 : ab224250 at 0.4 µg/ml
Lane 2 : Anti-OXCT1/SCOT antibody (ab224250) at 0.4 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) lysate
Lane 2 : A549 (Human lung carcinoma cell line) lysate
Predicted band size: 56 kDaLoading control: Anti-COX4I1.
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PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for OXCT1/SCOT (green) using ab224250 (4 µg/ml) in ICC/IF.
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All lanes : Anti-OXCT1/SCOT antibody (ab224250) at 1/100 dilution
Lane 1 : NIH/3T3 (mouse embyro fibroblast cell line) cell lysate
Lane 2 : NBT-II (rat Wistar bladder tumor cell line) cell lysate
Predicted band size: 56 kDa