Recombinant E. coli Beta lactamase TEM precursor protein (ab67672)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Active: Yes
- Suitable for: SDS-PAGE
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Product name
Recombinant E. coli Beta lactamase TEM precursor protein -
Biological activity
Specific Activity: 1,500IU/mg. Unit Definition: One unit will hydrolyze 1.0 µmole of indicated substrate per min at pH 7.0 at 25 ° C. The International Unit (using benzylpenicillin as substrate) is approximately equal to 600 Levy or 75 Pollock units. -
Purity
> 90 % SDS-PAGE.
Purity: Greater than 90.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. -
Expression system
Escherichia coli -
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Escherichia coli -
Sequence
MHPETLVK VKDAEDQLGA RVGYIELDLN SGKILESFRP EERFPMMSTF KVLLCGAVLS RVDAGQEQLG RRIHYSQNDL VEYSPVTEKH LTDGMTVREL CSAAITMSDN TAANLLLTTI GGPKELTAFL HNMGDHVTRL DRWEPELNEA IPNDERDTTM PAAMATTLRK LLTGELLTLA SRQQLIDWME ADKVAGPLLR SALPAGWFIA DKSGAGERGS RGIIAALGPD GKPSRIVVIY TTGSQATMDE RNRQIAEIGA SLIKHW -
Additional sequence information
Beta-Lactamase TEM precursor Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 263 amino acids and having a molecular mass of 29 kDa.
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Preparation and Storage
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Alternative names
- Beta-lactamase TEM
- blaT-6
- BLAT_ECOLX
see all -
Function
TEM-type are the most prevalent beta-lactamases in enterobacteria; they hydrolyze the beta-lactam bond in susceptible beta-lactam antibiotics, thus conferring resistance to penicillins and cephalosporins. TEM-3 and TEM-4 are capable of hydrolyzing cefotaxime and ceftazidime. TEM-5 is capable of hydrolyzing ceftazidime. TEM-6 is capable of hydrolyzing ceftazidime and aztreonam. TEM-8/CAZ-2, TEM-16/CAZ-7 and TEM-24/CAZ-6 are markedly active against ceftazidime. IRT-4 shows resistance to beta-lactamase inhibitors. -
Sequence similarities
Belongs to the class-A beta-lactamase family. - Information by UniProt
