Anti-OXCT1/SCOT antibody (ab105320)
Key features and details
- Rabbit polyclonal to OXCT1/SCOT
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-OXCT1/SCOT antibody
See all OXCT1/SCOT primary antibodies -
Description
Rabbit polyclonal to OXCT1/SCOT -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant fragment corresponding to Human OXCT1/SCOT aa 171-434. (BC009001)
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Positive control
- Human fetal heart and brain lysates; Human fetal kidney tissue.
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General notes
Protein previously labeled as OXCT1.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Properties
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Form
Lyophilized:Reconstitute in 200ul sterile water -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: 0.1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-OXCT1/SCOT antibody (ab105320) at 1/1000 dilution
Lane 1 : HEK293 whole cell lysate with SEA BLOCK blocking buffer
Lane 2 : Mitochondria isolated from HEK293 cells with SEA BLOCK blocking buffer
Lane 3 : Mitochondria isolated from non-synaptosomal human brain tissue. with SEA BLOCK blocking buffer
Lane 4 : Mitochondria isolated from synaptosomal human neural tissue. with SEA BLOCK blocking buffer
Lane 5 : Non-synaptosomal murine brain mitochondria (-ve control) with SEA BLOCK blocking buffer
Lane 6 : Synaptosomal murine neural mitochondria (-ve control) with SEA BLOCK blocking buffer
Lane 7 : STHdh-Q7/Q7 mitochondria (-ve control) with SEA BLOCK blocking buffer
Lane 8 : STHdh-Q111/Q111 mitochondria (-ve control) with SEA BLOCK blocking buffer
Lysates/proteins at 20 µg per lane.
Blocking peptides at 100 % per lane.
Secondary
All lanes : Goat anti-rabbit IRDye® 800CW at 1/30000 dilution
Performed under reducing conditions.
Predicted band size: 56 kDa
Additional bands at: 20 kDa (possible non-specific binding), 25 kDa (possible non-specific binding), 52 kDa (possible post-translational modification), 70 kDa (possible non-specific binding)
Exposure time: 5 minutes
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All lanes : Anti-OXCT1/SCOT antibody (ab105320) at 1/500 dilution
Lane 1 : Human fetal heart lysate
Lane 2 : Human fetal brain lysate
Predicted band size: 56 kDa
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ab105320 at 1/100 dilution staining OXCT1/SCOT in Human fetal kidney tissue